Abstract
Chemiluminescence of many biological systems, including plasma membranes, is due to exothermal reactions of oxidizing processes of the lipids and other cell components /Greenwood, 1982, Halliwell, 1985 /. When biological preparations are placed in an electric field the rate of peroxide processes increases several times and the intensity of luminescence increases strongly. Electrochemiluminescence /ECL/ was used in organic chemistry studies /Akins, 1979/. Successful attempts have been made for application of this method in the investigation of peroxide and free-radical processes in biology and medicine /Bard, 1972, Orel, 1976/.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Akins, D.G. and Barke, R.G., 1979, Energy Transfer in Reaction of Electrogenerated Aromatic Anions and Benzoyl Peroxide Chemiluminescence and its Mechanism, Chem. Phys. Lett., 29/3/: 428–435.
Bard, A.I., Keszthelyi, C.P. and Jachikawa, H., 1972, On theEfficiency of Electrogenerated Chemiluminescence, in:Chemiluminescence and Bioluminescense, Proc.Int.Symp.Athens,1972, N.Y.-L.
Barenholz, Y. and Gatt, S., 1982, in: Phoapholipids, Elsevier, North Holland
Folch, J., Lees, M. and Sloane-Stanley, G.H., 1957, A Simple Method for the Isolation and Purification of Total Lipids from Animal Tissues, J. Biol. Chem., 266: 497–509
Greenwood, C., Hill, H.A.O., 1982, in: Oxygen in Biological Systems, Chemy Br., London
Halliwell, B., Cutteridge, J.M.C., 1985, in: Free Radicals in Biology and Medicine, Oxford University Press, Oxford
Harman, D., 1968, Free Radical Theory of Ageing.Effects of Free Radical Reaction Inhibitors on the Mortality role of Male LAF mice, J. Gerontology, 23: 476–482
Kahovkova, J. and Odavich, R., 1969, A Simple Method for Analysis of Phospholipids Separated on Thin-layer Chromatography, J. Chromatogr., 40: 90–95
Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall,R.J., 1951, Protein Measurement with the Folin Phenol Reagent, J. Biol. Chem., 193: 265–275
Orel, V.E., 1976, Registration Method of Blood Plasm Electrochemiluminescence- in: Utilisation of Radioelectronics in the Field of Medical and Biological Studies, Naukova Dumka, Kiev /In Russian/ 135–156
Rothstein, M., 1982, in: Biochemical Approaches to Aging, Acad.Press, New York
Sperry, W. and Web, M., 1957, A Revision of Shoenheimer- Sperry Method for Determination, J. Biol. Chem., 187: 97–106
Tappel, A.L., 1973, Lipid Peroxidation Damage to Cell Components, Fed. Proc. 32/8/: 1870–1874
Yeagle,P.L., 1985, Cholesterol and the Cell Membrane, Biochim. Biophys. Acta, 822: 267–287
Zlatanov, I.V. and Smilenov, L.B., 1986, The Effect of Age on the Activities of Membrane-Bound 5-nucleotidase, Guanylate Cyclase and Adenylate Cyclase in Rat Testes, Compt. Rend. Acad. Bulgare Sci., 39: 101–104
Zs.-Nagy, I., 1985, Aging of the Cellular Membrane: Basic Principles and Pharmacological Interventions, Geriatrika, 1/8/:102–111
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Plenum Press, New York
About this chapter
Cite this chapter
Zlatanov, I., Bachev, S. (1988). The Electrochemiluminescent Characteristics Correlate to the Age-Dependent Changes in the Composition of Plasma Membranes. In: Markov, M., Blank, M. (eds) Electromagnetic Fields and Biomembranes. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9507-6_17
Download citation
DOI: https://doi.org/10.1007/978-1-4615-9507-6_17
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4615-9509-0
Online ISBN: 978-1-4615-9507-6
eBook Packages: Springer Book Archive