Abstract
The murine coronaviruses contain two different classes of glycoproteins (for review see 1). The envelope glycoprotein E2 resembles in its composition and biosynthetic processing the classical type of viral glycoprotein: E2 acquires N-glycosidically linked carbohydrate side chains in a cotranslational event at the rough endoplasmic reticulum (RER) 2) and in addition, it contains covalently linked fatty acids 3). The 180K species of E2 is processed posttranslationally by proteolytic cleavage into two 90K species 2,4).
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Keywords
- High Performance Liquid Chromatography
- Rough Endoplasmic Reticulum
- Viral Glycoprotein
- Carbohydrate Side Chain
- Synthetic Primer
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References
L. S. Sturman and K. V. Holmes (1983) The molecular biology of coronaviruses, in: Adv. in Virus Research 28, in press.
H. Niemann, B. Boschek, D. Evans, M. Rosing, T. Tamura, and H.-D. Klenk (1982) Post-translational glycosylation of coronavirus glycoprotein E1: inhibition by monensin. The EMBO J. 1, 1499–1504.
H. Niemann and H.-D. Klenk (1981) Coronavirus glycoprotein E1, a new type of viral glycoprotein. J. Mol. Biol. 153, 993–1010.
K. V. Holmes, E. W. Doller and J. N. Behnke (1981) Analysis of the functions of coronavirus glycoproteins by differential inhibition of synthesis with tunicamycin, in: Biochemistry and Biology of Coronaviruses, Adv. in Exp. Med. and Biol. Vol. 142, pp.133–142, V. ter Meulen, S. Siddell, and H. Wege, eds., Plenum-Press, New York, London.
L. S. Sturman (1981) The structure and behavior of Coronavirus A59 glycoproteins, in: Biochemistry and Biology of Coronaviruses. Adv. in Exp. Med. and Biology Vol. 142, p. 1., V. ter Meulen, S. Siddell, and H. Wege, eds., Plenum-Press, New York.
E.W. Doller and K. V. Holmes (1980) Different intracellular transportation of the envelope glycoproteins E1 and E2 of coronavirus MHV. Abstracts of the American Society of Microbiology, p. 267.
L. S. Sturman (1977) Characterization of a coronavirus. I. Structural proteins: effects of preparative conditions on the migration of protein in Polyacrylamide gels. Virology 77, 637.
L. S. Sturman, K. V. Holmes and J. Behnke (1980) Isolation of coronavirus envelope glycoproteins and interaction with the viral nucleocapsid. J. Virol. 33, 449.
R. Geyer, H. Geyer, S. Kühnhardt, W. Mink and S. Stirm (1983) Methylation analysis of complex carbohydrates in small amounts: Capillary gas chromatography-mass fragmentography of methyl-alditol acetates obtained from N-glycosidically linked glycoprotein oligosaccharides. Anal. Biochem. 133, in press.
M. J. Gait, H. W. D. Matthes, M. Singh, B. S. Sproat and R. Titmas (1982) Rapid synthesis of oligodeoxynucleotides VII. Solid phase synthesis of oligodeoxyribonucleotides by a continous flow phosphotriester method on Kieselguhr-polyamide support. Nucl. Acids Res. 10, 6243.
R. Frank, D. Müller, and C. Wolff (1981) Identification and suppression of secondary structures formed from deoxynucleotides during electrophoresis in denaturing Polyacrylamide gels. Nucl. Acids Res. 9, 4967.
H. Land, M. Grez, H. Hauser, W. Lindenmaier and G. Schütz (1981) 5′-Terminal sequences of eucaryotic mRNA can be cloned with high efficiency. Nucl. Acids Res. 9, 2251.
U. Rüther (1982) pUR250 allows rapid chemical sequencing of both DNA strands of its inserts. Nucl. Acids Res. 10, 5765.
M. Dagert and S. D. Ehrlich (1979) Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene 6, 23.
M. Grunstein and H. Hogness (1975) Colony hybridization: A method for the isolation of cloned DNAs that contain a specific gene. Proc. Natl. Acad. Sci. USA 72, 3961.
L. Dente, G. Cesareni and R. Cortese (1983) pEMBL: a new family of single stranded plasmids. Nucl. Acids Res. 11, 1645.
H. Garoff and W. Ansorge (1981) Improvement of DNA-sequencing gels. Anal. Biochem. 115 450–457.
R. Kornfeld and S. Kornfeld (1980) Structure of glycoproteins and their oligosaccharide units, in: The Biochemistry of Glycoproteins and Proteoglycans, W. J. Lennarz, ed., Plenum-Press, New York, London, p. 1–34.
R. E. Feaney and Y. Yeh (1978) Advances in protein Chemistry 32, 192–282.
J. Armstrong, S. Smeekens and P. Rottier (1983) Sequence of the nucleocapsid gene from murine coronavirus MHV-A59. Nucl. Acids Res. 11, 883–891.
M. M. C. Lai, C. D. Patton and S. A. Stohlman (1982) Further characterization of mouse hepatisis virus: presence of common 5′end nucleotides. J. Virol. 41, 557–565.
A. M. Maxam and W. Gilbert (1980) Sequencing end-labelled DNA with base-specific chemical cleavages. Methods Enzymol. 65, 499.
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Niemann, H., Heisterberg-Moutsis, G., Geyer, R., Klenk, HD., Wirth, M. (1984). Glycoprotein E1 of MHV-A59: Structure of the 0-Linked Carbohydrates and Construction of Full Length Recombinant cDNA Clones. In: Rottier, P.J.M., van der Zeijst, B.A.M., Spaan, W.J.M., Horzinek, M.C. (eds) Molecular Biology and Pathogenesis of Coronaviruses. Advances in Experimental Medicine and Biology, vol 173. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9373-7_20
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