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Regulation of Fibroblast Growth by Multiple Growth Factors in Serum-Free Media

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Mammalian Cell Culture

Abstract

In vitro studies of cellular growth regulation are based on observations that monolayer cultures of cell lines derived from normal tissue reversibly arrest growth at high density (Todaro and Green, 1963; Todaro et al., 1965). What originally made this model of growth control particularly interesting were demonstrations that agents that induced tumors in vivo also induced overgrowth of quiescent cell monolayers into piles of cells called foci. Early demonstrations of In vitro transformation included studies of carcinogenic hydrocarbons (Chen and Heidelberger, 1969), Rous sarcoma virus (Temin and Rubin, 1958), and the DNA1 tumor viruses SV40 (Todaro and Green, 1964), and polyoma (Stoker and MacPherson, 1961). Thus, an apparent correlation existed between aberrant growth in monolayer cultures and aberrant growth of tissue in vivo. The exact relationship between the properties of transformed cell lines In vitro and the invasive and metatastic behavior of tumors in vivo is not resolved. However, the cellular changes that permit growth of tumors cells in vivo and In vitro under conditions normaly not suited for cell proliferation are likely to be related.

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  1. Laboratory of Neoplastic Disease Mechanisms, Dana-Farber Cancer Institute, Boston, Massachusetts, 02115, USA

    Paul V. Cherington

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    Jennie P. Mather

  2. The Rockefeller University, New York, New York, USA

    Jennie P. Mather

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Cherington, P.V. (1984). Regulation of Fibroblast Growth by Multiple Growth Factors in Serum-Free Media. In: Mather, J.P. (eds) Mammalian Cell Culture. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9361-4_2

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