Abstract
Our interest in follicle stimulating hormone binding inhibitors (FSH-BI) stemmed from earlier studies on the presence of a low molecular weight testicular factor(s) in buffer or water extracts of mature rat testis which inhibited binding of 125I-FSH to gonadal receptors (Reichert and Abou-Issa, 1977). Shortly thereafter, we described the presence of similar factors in human serum (Reichert et al., 1979) and in bovine follicular fluid (Darga and Reichert, 1978). Most recently, low molecular weight components with FSH-BI activity have been detected in human seminal plasma (Dias et al., 1981). Our initial studies on these factors have been summarized in several reviews (Reichert, 1978; Reichert et al., 1981) and will not be discussed further at this time. Rather, we will present in this report a description of our latest efforts to characterize the FSH binding inhibitors found in human plasma and bovine follicular fluid.
This work was supported by United States Department of Agriculture Cooperative Agreement 58-3244-0-158 and a Grant from the Andrew W. Mellon Foundation.
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References
Abou-Issa, H., and Reichert, L. E., Jr., 1976, Properties of the fol-litropin receptor interaction: Characterization of the interaction of follitropin with receptors in purified membranes isolated from mature rat testes tubules, J. Biol. Chem., 251:3326.
Birnbaumer, L., Yang, P., Hunzicker-Dunn, M., Bockaert, J., and Duran, J. M., 1976, Adenylyl cyclase activities in ovarian tissues. I. Homogenization and conditions of assay in graafian follicles and corpora lutea of rabbits, rats, and pigs: Regulation by ATP, and Some Comparative Properties, Endocrinology, 99:163.
Darga, N. C., and Reichert, L. E., Jr., 1978, Some properties of the interaction of follicle stimulating hormone with bovine granulosa cells and its inhibition by follicular fluid, Biol. Reprod., 19:235.
Darga, N. C.,and Reichert, L. E., Jr., 1979, Evidence for the presence of a low molecular weight binding inhibitor in bovine follicular fluid, in: “Ovarian, Follicular, and Corpus Luteum Function,” C. P. Channing, J. M. Marsh, and W. A. Sadler, eds., pp. 338–342, Plenum Press, New York.
Dias, J. A., Treble, D. H., Bennet, A. H., and Reichert, L. E., Jr., 1981, Follitropin receptor binding inhibitors in human seminal plasma, Andrology, 5:259.
Hanson, K. R., Ling, R., and Hovir, E., 1967, A computer program for fitting data to the Michaelis-Menton equation, Biochem. Biophys. Res. Commun., 29:194.
Jordan, A. W., Caffrey, J. L., and Niswender, G. D., 1978, Catecholamine- induced stimulation of progesterone and adenosine 3r,5f-monophosphate production by dispersed ovine luteal cells, Endocrinology, 103:385.
Lee, C. Y., and Takahashi, H., 1977, Follicle stimulating hormone receptors in rat ovaries; Decrease in numbers of binding sites associated with luteinization, Endocrinology, 101:869.
Reichert, L. E., Jr., 1978, Some properties of small molecular weight tissue and serum inhibitors of follitropin binding to receptor, in: “Novel Aspects of Reproductive Physiology,” C. H. Spilman and J. W. Wilks, eds., pp. 355–368, Academic Press, New York.
Reichert, L. E., Jr., and Abou-Issa, H., 1977, Studies on a low molecular weight testicular factor which inhibits binding of FSH to receptor, Biol. Reprod., 17:614.
Reichert, L. E., Jr., Sanzo, M. A., and Darga, N. S., 1979, Studies on a low molecular weight follicle stimulating hormone binding inhibitor from human serum, J. Clin. Endocrinol. Metab., 49: 866.
Reichert, L. E., Jr., Sanzo, M. A., and Dias, J. A., 1981, Studies on purification and characterization of gonadotropin binding inhibitors and stimulators from human serum and seminal plasma, in: “Intragonadal Regulators of Reproduction,” P. P. Franchimont and C. P. Channing, eds., Academic Press, London, in press.
Steiner, A. L., Parker, C. W., Kijnis, D. M., 1972, Radioimmunoassay for cyclic nucleotides. I. Preparation of antibodies and iodinated cyclic nucleotides, J. Biol. Chem., 247:1106.
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© 1982 Plenum Press, New York
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Reichert, L.E., Sanzo, M.A., Fletcher, P.W., Dias, J.A., Lee, C.Y. (1982). Properties of Follicle Stimulating Hormone Binding Inhibitors Found in Physiological Fluids. In: Channing, C.P., Segal, S.J. (eds) Intraovarian Control Mechanisms. Advances in Experimental Medicine and Biology, vol 147. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9278-5_8
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DOI: https://doi.org/10.1007/978-1-4615-9278-5_8
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