A Photochemical Characterization of Reactions of Psoralen Derivatives with DNA
Psoralens are nucleic acid photoreagents which form mono-and diadducts to pyrimidine bases upon irradiation with long wavelength ultraviolet light (Musajo et al., 1967). Diaddition results in the formation of interstrand bridges or crosslinks in nucleic acid dou ble helices (Cole, 1970, 1971). Crosslink formation is a multistep process involving initial dark binding or intercalation of the psoralen, photoaddition of the psoralen to a pyrimidine base forming a monoadduct followed by conversion of the monoadduct to crosslink by a second photoaddition to a second pyrimidine base. Each of these steps may be investigated as a function of the type and position of the substituents of a given psoralen compound.
KeywordsQuantum Yield Photochemical Reaction Pyrimidine Base Suction Filtration Pyridinium Chloride
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