Abstract
The physiological role of PTH and particularly its renal effects have been extensively studied in the rat. However, since PTH is rapidly degraded in the presence of rat renal tissue (1), the first step of its action, namely binding to specific receptors, has mainly been studied with chicken or bovine renal membranes (2–5). The only binding studies using membranes from murine source were reported by Malbon and Zull (6), but were limited to a single incubation time and a single concentration of labelled PTH. Moreover, the physiological control of PTH binding to its receptors and particularly the role of plasma PTH have not been till now analyzed. We have recently prepared a biologically active tritiated derivative of the synthetic 1–34 fragment of human PTH (7). Furthermore, we have demonstrated that this tracer specifically binds to renal membranes (7) and to glomeruli (8) isolated from rat renal cortex. The present paper extends our earlier results obtained with rat renal membranes and demonstrates that PTH binding and PTH-sensitive adenylate cyclase activity are inversely related to plasma PTH concentration.
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References
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© 1980 Plenum Press, New York
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Loreau, N., Lajotte, C., Lafaye, M., Ardaillou, R. (1980). Renal Receptors for Parathyroid Hormone in Normal, Parathyroidectomized and Vitamin D-Deficient Rats. In: Massry, S.G., Ritz, E., Jahn, H. (eds) Phosphate and Minerals in Health and Disease. Advances in Experimental Medicine and Biology, vol 128. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9167-2_58
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DOI: https://doi.org/10.1007/978-1-4615-9167-2_58
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