Lymphocyte Production and Organ Distribution of Newly Formed Lymphocytes after Selective 3H-Deoxycytidine Labelling of Mesenteric Lymph Nodes
Systemic labelling procedures with DNA precursors showed lymphocytopoiesis in normal lymph nodes. For quantitative aspects of lymphocyte production in lymph nodes, the rate of exit from the lymph nodes and entry of newly formed lymphocytes from other organs must be known. For rat lymphatic tissue, a preferential labelling of lymphocytes with a rapid turnover rate by tritiated deoxycytidine (3HdCy) application was demonstrated (1). In the present study mesenteric lymph nodes of young pigs were selectively labelled with 125 μCi (specific activity 20 Ci/mM) of 3HdCy. The organ distribution of radioactively labelled lymphocytes one or two days later indicated the migration pattern of newly formed lymph node lymphocytes, and by summing up all labelled lymphocytes the total amount of lymphocytopoiesis could be estimated. In normal young pigs (about 23 kg body weight) mesenteric lymph nodes were labelled by about 50 minute injections of 3HdCy into the parenchyma. Two pigs were killed 24 and two 48 hours after labelling by bleeding to death.
KeywordsLymph Node Labelling Index Mesenteric Lymph Node Inguinal Lymph Node Organ Distribution
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