Immunochemical and Compositional Comparison of Cytochrome P-450cam of Pseudomonas Putida and P-450LM of Phenobarbital-Induced Rabbit Liver Microsomes
During the last two years my laboratory has been concerned with the determination of the structure and sequence of cytochrome P-450cam of Pseudomonas putida. One of our more recent lines of investigations is to compare this hemeprotein to P-450 cytochrome of higher organisms. The main objective of this investigation is to identify characteristic structural features common to P-450 type hemeproteins, and to demonstrate the value of the readily soluble and accessible, pure cytochrome P-450cam as a structural model.
KeywordsPseudomonas Putida Cyanogen Bromide Competitive Binding Assay Characteristic Structural Feature Additional Amino Acid Residue
Unable to display preview. Download preview PDF.
- Dus, K., Miguel, A. G., Smith, P. C. Litchfield, W. J., and Harrison, J. E. 1973. Hemepeptides Derived from Cytochrome P-450cam. 9th International Congress of Biochemistry, Stockholm, Abstracts, p. 341.Google Scholar
- van der Hoeven, T. A., and Coon, M. J. 1974. Preparation and Properties of Partially Purified Cytochrome P-450 and NADPH-cytochrome P-450 Reductase from Rabbit Liver Microsomes. J. Biol. Chem. 249: in press.Google Scholar