Drug Metabolism in Isolated Rat Liver Cells
Although considerable knowledge has been gathered on the functional aspects of microsomal monooxygenation, comparatively little has so far been known about the intracellular regulation of this process. For such studies, we have found the isolated rat liver cell system to be a very useful model, combining the convenience of an in vitro system with the access to the complex mechanisms of the intact in vivo system. This model has the advantage over the perfused liver that it readily lends itself to the study of rapid reaction sequences and makes quantitation of short-term drug metabolic reactions easier. It is also superior to liver slices which often show considerable leakage of adenine and pyridine nucleotides and where substrate penetration and oxygen diffusion may present problems depending on the relative thickness of the slice.
KeywordsDrug Metabolism Liver Microsome Spectral Change Isolate Liver Cell Sample Cuvette
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