Abstract
The interaction of monolayers of stearate and oleate with Ca++ and H+ have been studied in a Tris-buffered system where trans-surface potential (ΔV) and surface accumulation of Ca45 per mole lipid (θ) have been studied. ΔV-area data suggest that the orientation of the carbonyl dipole is the same for both lipids when the monolayers are close-packed. θ is higher for stearate than for oleate at bulk calcium concentrations in the range 10−7 to 10−4 M, but for both lipids it is far greater than would be expected from a simple mass-law relationship using the bulk affinity constant. Both the titration curves and the Ca45 accumulation data can be fitted reasonably well by a Donnan model which takes monolayer charge into account.
Supported in part by USPHS grants HE-06285, HE-5251 and FR-00122
American Heart Association Established Investigator 1967–1972
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Bibliography
Danielli, J.F., Proc. Roy. Soc. Lond., Ser. B, 1937, 122, 155.
Danielli, J.F., J. Exptl. Biol., 1944, 20, 167.
Danielli, J.F. and J.T. Davies, Adv. in Enzymology, 1951, 11, 35.
Stern, O., Z. Elektrochem., 1924, 30, 508.
Goldstein, G., Nucleonics, 1965, 23, 67.
Goddard, E.D., and J.A. Ackilli, J. Coll. Sci., 1963, 18, 585.
Spink, J.A., J. Coll. Sci., 1963, 18, 512.
Garvin, J.E., and M.L. Karnovsky, J. Biol. Chem., 1956, 221, 211.
Gouy, M., J. de Phys., Ser. 4, 1910, 9, 457.
Chapman, D.L., Phil. Mag., 1913, 25, 475.
Deamer, D.W., and D.G. Cornwell, Biochim. Biophys. Acta, 1966, 116, 555.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1970 Plenum Press, New York
About this chapter
Cite this chapter
Goerke, J., Harper, H.H., Borowitz, M. (1970). The Interaction of Calcium with Monolayers of Stearic and Oleic Acid. In: Blank, M. (eds) Surface Chemistry of Biological Systems. Advances in Experimental Medicine and Biology, vol 7. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9005-7_2
Download citation
DOI: https://doi.org/10.1007/978-1-4615-9005-7_2
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4615-9007-1
Online ISBN: 978-1-4615-9005-7
eBook Packages: Springer Book Archive