Abstract
The purposes of this chapter are to introduce the teratologist to the vast field of cell, tissue, and organ culture and highlight the potential uses of these scientific approaches. Although in the strict sense the study of teratology implies environmental agents, the effect of mutant genes in development is so obviously related that some consideration will be given to the latter area in this discussion. Technical advances in these areas now offer a relatively simple tool for the study and analysis of isolated teratologic events. With the use of special plastics, premixed media, and recent literature descriptions, these techniques no longer have a mystical flavor and are certainly not out of the reach of the laboratory investigator in teratology.
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References
Adams, C. E., Hay, M. F., and Lutwak-Mann, C., 1961, The action of various agents upon the rabbit embryo, J. Embryol. Exp. Morphol. 9:468.
Amprino, R., 1965, Aspects of limb morphogenesis in the chicken, in: Organogenesis (R. L. De-Haan and H. Ursprung, eds.), pp. 255–281, Holt, Rinehart and Winston, New York.
Artzt, K., Dubois, P., Bennett, D., Condamine, H., Babinet, C., and Jacob, F., 1973, Surface antigens common to mouse cleavage embryos and primitive teratocarcinomas in culture, Proc. Natl. Acad. Sci. U.S. 71:811.
Auerbach, R., 1965, Experimental analysis of lymphoid differentiation in the mammalian thymus and spleen, in: Organogenesis (R. L. DeHaan and H. Ursprung, eds.), pp. 539–557, Holt, Rinehart and Winston, New York.
Aydelotte, M. B., 1965, Respiratory tract, in: Cells and Tissues in Culture: Methods, Biology, and Physiology (E. N. Willmer, ed.), Vol. 2, pp. 567–606, Academic Press, New York.
Aydelotte, M. B., and Kochhar, D. M., 1972, Development of mouse limb buds in organ culture: chondrogenesis in the presence of a proline analog l-azetidine-2-carboxylic acid, Develop. Biol. 28:191.
Bergman, G. J., Mackler, B., and Shepard, T. H., 1972, Studies of oxidative energy deficiency. I. Achondroplasia in the rabbit, Arch. Biochem. Biophys. 150:137.
Bernfield, M. R., and Wessels, N. K., 1970, Intro- and extracellular control of epithelial morphogenesis, Develop. Biol. Suppl. 4:195.
Biggers, J. D., 1965, Cartilage and bone, in: Cells and Tissues in Culture: Methods, Biology, and Physiology (E. N. Willmer, ed.), Vol. 2, pp. 195–260, Academic Press, New York.
Blanchard, M., and Josso, N., 1974, Source of the anti-mullerian hormone synthesized by fetal testis: mullerian-inhibiting activity of fetal bovine sertoli cells in tissue culture, Pediat. Res. 8:968.
Blandau, R. J., ed., 1971, The Biology of the Blastocyst, Univ. of Chicago Press, Chicago.
Brinster, R. L., 1973, Nutrition and metabolism of the ovum, zygote, and blastocyst, in: Handbook of Physiology (R. O. Greep, ed.), pp. 165–215, Williams & Wilkins, Baltimore, and American Physiological Society, Washington, D.C.
Caplan, A. I., 1970, Effects of nicotinamide-sensitive teratogen 3-acetylpyridine on chick limb cells in culture, Exp. Cell Res. 62:341.
Caplan, A. I., 1972, The site and sequence of action of 6-aminonicotinamide in causing bone malformations of embryonic chick limb and its relationship to normal development, Develop. Biol. 28:71.
Carter, S. B., 1967, Effects of cytochalasins on mammalian cells, Nature 213:261.
Cassiman, J. J. and Bernfield, M. R., 1974, Morphogenetic properties of human embryonic cells: aggregation of dissociated cells and histogenesis in cultured aggregates, Pediat. Res. 8:184.
Chalkley, H. W., 1943–1944, Method for quantitative morphologic analysis of tissues, J. Natl. Cancer Inst. 4:47.
Chaube, S., and Murphy, M. L., 1969, The teratogenic effects of the recent drugs active in cancer chemotherapy, in: Advances in Teratology (D. H. Woollam, ed.), Vol. 3, Academic Press, New York.
Chu, E. H. Y., 1971, Induction and analysis of gene mutations in mammalian cells in culture, in: Chemical Mutagens Principles and Methods for Their Detection (A. E. Hollaender, ed.), Vol. 2, pp. 411–444, Plenum Press, New York.
Clayton, R. M., 1976, A Rapidin Vitro Assay System for Teratogenic Compounds in Tests of Teratogenicity in Vitro (J. D. Ebert and M. Marois, eds.), pp. 473–483, North-Holland, Amsterdam.
Coulombre, A. J., 1965, The eye, in: Organogenesis (R. L. DeHaan and H. Ursprung, eds.), pp. 219–251, Holt, Rinehart and Winston, New York.
Crocker, J. F. S., and Vernier, R. L., 1970, Fetal kidney in organ culture: abnormalities of development induced by potassium, Science 169:485.
Cutter, E. G., 1967, Surgical techniques in plants, in: Methods in Developmental Biology (F. H. Wilt and N. K. Wessels, eds.), pp. 623–634, Thomas Y. Crowell, New York.
Daniel, J. C., 1971, Methods in Mammalian Embryology, W. H. Freeman, San Francisco.
DeHaan, R. L., 1965, Morphogenesis in the vertebrate heart, in: Organogenesis (R. L. DeHaan and H. Ursprung, eds.), pp. 377–419, Holt, Rinehart and Winston, New York.
Donahue, R. P., 1975, Chromosomal anomalies and the meiotic divisions of the oocyte, in: Methods for Detection of Environmental Agents Which Produce Congenital Defects; Proceedings of the Guadeloupe Conference Sponsored by L’Institut de la Vie (T. H. Shepard, J. R. Miller, and M. Marois, eds.), pp. 125–139, North-Holland, Amsterdam.
Dulak, N. C., and Temin, H. M., 1973, A partially purified polypeptide fraction from rat liver cell conditioned medium with multiplication-stimulating activity for embryo fibroblasts, J. Cell. Physiol 81:153, 161.
Ede, D. A., and Agerbak, G. S., 1968, Cell adhesion and movement in relation to the developing limb pattern in normal and talpid 3 mutant chick embryos, J. Embryol. Exp. Morphol. 20:81.
Fell, H. B., 1961, The influence of hydrocortisone on the action of excess vitamin A on limb bone rudiments in culture, J. Exp. Med. 114:343.
Fell, H. B., and Rinaldini, L. M., 1965, The effects of vitamin A and C on cells and tissues, in: Cells and Tissues in Culture: Methods, Biology, and Physiology (E. N. Willmer, ed.), Vol. 1, pp. 659–699, Academic Press, New York.
Ferm, F. H., 1971, Permeability of the mammalian blastocyst to teratogens, in: The Biology of the Blastocyst (R. J. Blandau, ed.), pp. 291–302, Univ. of Chicago Press, Chicago.
Fraser, R. A., and Goetinek, P. F., 1971, Reduced synthesis of chondroitin sulphate by cartilage from the mutant nanomelia, Biochem. Biophys. Res. Commun. 43:494.
Gaillard, P. J., and Schaberg, A., 1965, Endocrine glands, in: Cells and Tissues in Culture: Methods, Biology, and Physiology (E. N. Willmer, ed.), Vol. 2, pp. 631–695, Academic Press, New York.
Gartler, S. M., 1967, Genetic markers as tracers in cell culture, Natl. Cancer Inst. Monogr. 26:167.
Gartler, S. M., and Farber, R. A., 1973, Biochemical identification of cells in culture, in: Tissue Culture Methods and Applications (P. F. Kruse, Jr. and M. K. Patterson, Jr., eds.), pp. 797–805, Academic Press, New York.
Gartler, S. M., and Pious, D. A., 1966, Genetics of mammalian cell cultures, Humangenetik 2:83.
Gitlin, D., and Biasucci, A., 1969, Ontogenesis of immunoreactive growth hormone, follicle stimulating hormone, thyroid stimulating hormone, luteinizing hormone, chorionic, prolactin, and chorionic gonadotropin in the human conceptus, J. Clin. Endocrinol. Metab. 29:926.
Grobstein, C., 1965, Differentiation: environmental factors, chemical and cellular, in: Cells and Tissues in Culture: Methods, Biology, and Physiology (E. N. Willmer, ed.), Vol. 1, pp. 463–488, Academic Press, New York.
Hilfer, S. R., 1962, The stability of embryonic chick thyroid cells in vitro as judged by morphological and physiological criteria, Develop. Biol. 4:1.
Houck, J. C., and Cheng, R. F., 1973, Isolation, purification, and chemical characterization of the serum mitogen for diploid human fibroblasts, J. Cell. Physiol. 8:257.
Johnson, D. R., 1974, The in vivo behavior of achondroplastic cartilage from cartilage anomaly (can/can) mouse, J. Embryol. Exp. Morphol. 31:313.
Josso, N., 1974, Mullerian-inhibiting activity of human fetal testicular tissue deprived of germ cells by in vitro irradiation, Pediat. Res. 8:755.
Jost, A., 1972, A new look at the mechanisms controlling sex differentiation in mammals, Johns Hopkins Med. J. 130:38.
Kasten, F. H., 1973, Mammalian myocardial cells, in: Tissue Culture Methods and Applications (P. F. Kruse, Jr., and M. K. Patterson, Jr., eds.), pp. 72–81, Academic Press, New York.
Kochhar, D. M., and Aydelotte, M. B., 1974, Susceptible stages and abnormal morphogenesis in the developing mouse limb, analyzed in organ culture after transplacental exposure to vitamin A (retinoic acid), J. Embryol. Exp. Morphol. 31:721.
Konyukhov, B. V., and Bugrilova, R. S., 1968, The growth inhibiting factor in embryos of mutant stock brachypodism-H mice, Folia Biol. (Prague) 14:65.
Kruse, P. F., Jr., and Patterson, M. K., Jr., eds., 1973, Tissue Culture Methods and Applications, Academic Press, New York.
Mackler, M., Haynes, B., Inamdar, A. R., Pedegrand, L. R., Hall, J. G., and Cohen, M. M., 1973, Oxidative energy deficiency. II. Human achondroplasia, Arch. Biochem. Biophys. 159:885.
Macpherson, I., 1973, Microdrop techniques, in: Tissue Culture Methods and Applications (P. F. Kurse, Jr., and M. K. Patterson, Jr., eds.), pp. 241–244, Academic Press, New York.
Martin, G. M., 1973, Dilution plating on cover slip fragments, in: Tissue Culture Methods and Applications (P. F. Kruse, Jr., and M. K. Patterson, Jr., eds.), pp. 264–266, Academic Press, New York.
Martin, G. M., Sprague, C. A., and Epstein, C. J., 1970, Replicative life-span of cultivated human cells: effect of donor’s age, tissue, and genotype, Lab. Invest. 23:86.
McLaren, A., and Biggers, J. D., 1958, Successful development and birth of mice cultivated in vitro as early embryos, Nature 182:877.
Mintz, B., 1965, Experimental genetic mosaicism in the mouse, in: Preimplantation Stages of Pregnancy (G. E. W. Wolstenholme and M. O’Connor, eds.), pp. 194–216, Little, Brown, Boston.
Morris, J. M., Van Wagenen, G., Harteau, G. D., Johnston, D. W., and Carlsen, R. A., 1967, Compounds interfering with ovum implantation and development, Fertility Sterility 18:7.
Moscona, A. A., 1973, Cell aggregation, in: Cell Biology in Medicine (E. E. Bittar, ed.), pp. 571–591, John Wiley, New York.
Moscona, A. A., 1975, Embryonic cell and tissue cultures as test systems for teratogenic agents, in: Methods for Detection of Environmental Agents Which Produce Congenital Defects; Proceedings of the Guadeloupe Conference Sponsored by I’Tnstitut de la Vie (T. H. Shepard, J. R. Miller, and M. Marois, eds.), pp. 103–107, North-Holland, Amsterdam.
New, D. A. T., 1966, The Culture of Vertebrate Embryos, Academic Press, New York.
Paul, J. P., 1970, Cell and Tissue Culture, Williams & Wilkins, Baltimore.
Pious, D. A., 1975, Cell surfaces, genetics, and congenital malformations, J. Pediat. 68:162.
Pious, D. A., Hamburger, R. N., and Mills, S. E., 1964, Clonal growth of primary human cell cultures, Exp. Cell Res. 33:495.
Pious, D. A., Hawley, P., and Forrest, L. G., 1973, Isolation and characterization of HL-A variants of cultured human lymphoid cells, Proc. Natl. Acad. Sci. U.S. 70:1397.
Pious, D., Millis, A., and Sabo, K., 1975, In vitro cell growth rates and primary cellular growth deficiency syndromes, Pediatr. Res. 9:279 (Abstr.).
Prescott, D. M., 1964–1974, Methods in Cell Physiology, Vols. 1–8, Academic Press, New York.
Price, D., and Pannabecker, R., 1959, La différenciation du sexe chez l’embryon de rat. Comparaison entre le comportement des tractus genitaux et en culture, Arch. Anat. Microscop. Morphol. Exp. 48:223.
Rudland, P. S., Seifert, W., and Gospodarowicz, D., 1974, Growth control in cultured mouse fibroblasts: induction of the pleiotypic and mitogenic responses by a purified growth factor, Proc. Natl. Acad. Sci. U.S. 71:2600.
Rumery, R. E., Phinney, E., and Blandau, R. J., 1971, Culture of mammalian embryonic ovaries and oviducts, in: Methods in Mammalian Embryology (J. C. Daniel, ed.), pp. 472–495, W. H. Freeman, San Francisco.
Salzgeber, B., 1969, Étude comparative des effets de l’yperite azotée sur les constituants, mésodermique et ectodermique, des bourgeons de membres de l’embryon de poulet, J. Embryol. Exp. Morphol. 22:373.
Seegmiller, R., Ferguson, C. C., and Sheldon, H., 1972, Studies on cartilage. VI. A genetically determined defect in tracheal cartilage, J. Ultrastruct. Res. 38:288.
Shannon, J. E., and Macy, M. L., 1973, Freezing, storage, and recovery of cell stocks, in: Tissue Culture: Methods and Applications (P. F. Kruse, Jr., and M. K. Patterson, Jr., eds.), pp. 712–718, Academic Press, New York.
Shepard, T. H., 1967, Onset of function in the human fetal thyroid: biochemical and radioautographic studies from organ culture, J. Clin. Endocrinol. Metab. 27, 945.
Shepard, T. H., 1969, Growth and development of the human embryo and fetus, in: Endocrine and Genetic Diseases of Childhood (L. I. Gardner, ed.), pp. 1–6, W. B. Saunders, Philadelphia.
Shepard, T. H., 1971, Organ-culture studies of achondroplastic rabbit cartilage: evidence for a metabolic defect in glucose utilization, J. Embryol. Exp. Morphol. 25:347.
Shepard, T. H., and Bass, G. L., 1970, Organ culture of limb buds from riboflavin-deficient and normal rat embryos in normal and riboflavin deficient media, Teratology 3:163.
Shepard, T. H., Nelson, T., Oakley, G. P., and Lemire, R. J., 1971, A centralized laboratory for collection of human embryos and fetuses: seven years experience. I. Methods in monitoring of birth defects, in: Monitoring, Birth Defects, and Environment—The Problem of Surveillance (E. B. Hook, D. T. Janerich, and I. H. Porter, eds.), pp. 29–43, Academic Press, New York.
Shipman, C., Jr., 1973, Control of culture pH with organic buffers, in: Tissue Culture Methods and Applications (P. F. Kruse, Jr., and M. K. Patterson, Jr., eds.), pp. 709–712, Academic Press, New York.
Tarkowski, A. J., 1965, Embryonic and postnatal development of mouse chimeras, in: Preimplantation Stages of Pregnancy (G. E. Wolstenholme and M. O’Connor, eds.), pp. 183–193, Little, Brown, Boston.
Thorp, F. K., and Dorfman, A., 1967, Differentiation of connective tissues, in: Current Topics in Developmental Biology (A. A. Mascona, ed.), Vol. 2, pp. 151–190, Academic Press, New York.
Uchida, I. A., and Lee, V., 1974, Radiation-induced non-disjunction in mouse oocytes, Nature 250:601.
Willmer, E. N., ed., 1965, Cells and Tissues in Culture: Methods, Biology, and Physiology, Vols. 1 and 2, Academic Press, New York.
Wilson, J. G., 1973, Environment and Birth Defects, Academic Press, New York.
Wilt, F. H., and Wessells, N. K., eds., 1967, Methods in Developmental Biology, Thomas Y. Crowell, New York.
Wolff, E., 1965, Embryogenesis in vitro, in: Cells and Tissues in Culture: Methods, Biology, and Physiology (E. N. Willmer, ed.), Vol. 1, pp. 531–587, Academic Press, New York.
Wolff, E., and Keiny, M., 1957, Mise en évidence d’une action inhibitrice de l’extrait d’embryons de la race de poules ‘courte-pattes’ creeper sur la croissance des tibias cultivés in vitro, C. R. Acad. Sci. Paris 244:1089.
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Shepard, T.H., Pious, D. (1978). Cell, Tissue, and Organ Culture as Teratologic Tools. In: Wilson, J.C., Fraser, F.C. (eds) Research Procedures and Data Analysis. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-8939-6_3
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DOI: https://doi.org/10.1007/978-1-4615-8939-6_3
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