Abstract
Cummins (this publication) has given a broad review of the field of macromolecular diffusion as studied by photon correlation spectroscopy. In this article I will cover in more detail three aspects of the field: (1) Diffusion of identical, non-interacting macromolecules, spherically symmetric and/or small compared to the wavelength of light; (2) The effects and characterization of macromolecular polydispersity (non-identical macromolecules); and (3) The effects of inter-macromolecular interactions. The first topic is probably the simplest that can be conceived of under the heading of diffusion. As Cummins (this publication) has shown, the theory is straightforward, and I hope to convince you that experiments to date show adequate agreement with the theoretical predictions. From measurements on such systems one can obtain directly the translational diffusion coefficient D of the macromolecules. From D one can obtain, via the Einstein equation, the macromolecular frictional coefficient. For spherical macromolecules this yields through the Stokes equation the macromolecular radius. Also, combining D in the Svedberg equation with independently determined values for the sedimentation rate and partial specific volume one obtains the macromolecular weight.
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Pusey, P.N. (1974). Macromolecular Diffusion. In: Cummins, H.Z., Pike, E.R. (eds) Photon Correlation and Light Beating Spectroscopy. NATO Advanced Study Institutes Series, vol 3. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-8906-8_10
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DOI: https://doi.org/10.1007/978-1-4615-8906-8_10
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