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Methodology for Study of Isolated Perfused Rat Kidney in Vitro

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Renal Pharmacology

Abstract

The advantages of isolated perfused organs for biological study are several. Delivery and removal of substrates and products via normal vascular channels, and the absence of modifying effects from other organs are primary benefits. In the case of the kidney, a further advantage accrues from the fact that transport events transpire while the perfused tissue is being studied. For an organ in which the major energy expenditure is believed to be for transport (a process that occurs to only a limited extent in slices and not at all in homogenates), this is an important consideration.

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References

  • Bahlmann, J., Giebisch, G., Ochwadt, B., and Schoeppe, W. 1967. Micropuncture study of isolated perfused rat kidney. Am. J. Physiol., 212:77.

    PubMed  CAS  Google Scholar 

  • Bauman, A. W., Clarkson, T., and Miles, E. 1963. Functional evaluation of isolated perfused rat kidney. J. Appl. Physiol., 18:1239.

    PubMed  CAS  Google Scholar 

  • Bowman, R. H. 1966. Effects of diabetes, fatty acids, and ketone bodies on tricarboxylic acid cycle metabolism in the perfused rat heart. J. Biol. Chem., 241:3041.

    PubMed  CAS  Google Scholar 

  • Bowman, R. H. 1970. Gluconeogenesis in the isolated perfused rat kidney. J. Biol. Chem., 245:1604.

    PubMed  CAS  Google Scholar 

  • Bowman, R. H. 1975a. The perfused rat kidney. In: Methods in Enzymology, Vol. 39, pp. 3–11. Ed. by Hardman, J. G. and O’Malley, B. W. Academic Press, New York.

    Google Scholar 

  • Bowman, R. H. 1975b. Renal secretion of [35S]furosemide and its depression by albumin binding. Am.J. Physiol., 229:93.

    CAS  Google Scholar 

  • Bowman, R. H., and Maack, T. 1972. Glucose transport by the isolated perfused rat kidney. Am. J. Physiol., 222:1499.

    PubMed  CAS  Google Scholar 

  • Bowman, R. H., and Maack, T. 1974. Effect of albumin concentration and ADH on H2O and electrolyte transport in perfused rat kidney. Am. J. Physiol., 226:426.

    PubMed  CAS  Google Scholar 

  • Bowman, R. H., Dolgin, J., and Coulson, R. 1973. Furosemide, ethacrynic acid and iodoacetate on function and metabolism in perfused rat kidney. Am. J. Physiol., 224:416.

    PubMed  CAS  Google Scholar 

  • Broadus, A. E., Kaminsky, N. I., Hardman, J. G., Sutherland, E. W., and Liddle, G. W. 1970. Kinetic parameters and renal clearances of plasma adenosine 3′,5′-monophosphate and guanosine 3′,5′-monophosphate in man. J. Clin. Invest., 49:2222.

    Article  PubMed  CAS  Google Scholar 

  • Butlen, D., and Jard, S. 1972. Renal handling of 3′,5′-cyclic AMP in the rat. Pflügers Arch. Ges. Physiol., 331:172.

    Article  CAS  Google Scholar 

  • Coulson, R. 1976. Metabolism and excretion of exogenous adenosine 3′,5′-monophosphate and guanosine 3′,5′-monophosphate: studies in the isolated perfused rat kidney. J. Biol. Chem. 251:4958–4967.

    PubMed  CAS  Google Scholar 

  • Coulson, R., and Bowman, R. Hn 1974. Excretion and degradation of exogenous adenosine 3′,5′-monophosphate by isolated perfused rat kidney. Life Sci., 14:545.

    Article  PubMed  CAS  Google Scholar 

  • Coulson, R., Bowman, R. H., and Roch-Ramel, F. 1974. The effects of nephrectomy and probenecid on in vivo clearance of adenosine 3′,5′-monophosphate from rat plasma. Life Sci., 15:877.

    Article  PubMed  CAS  Google Scholar 

  • de Mello, G., and Maack, T. 1976. Nephron function of the isolated perfused rat kidney. Am. J. Physiol. 231:1699.

    PubMed  Google Scholar 

  • Krebs, H. A., and Henseleit, K. 1932. Untersuchungen über die Harnstoffbildung im Tierkörper. Z. Physiol, Chem., 210:33.

    Article  CAS  Google Scholar 

  • Little, J. R., and Cohen, J. J. 1974. Effect of albumin concentration on function of isolated perfused rat kidney. Am. J. Physiol., 226:512.

    PubMed  CAS  Google Scholar 

  • Maack, T., Johnson, V., Tate, S. S., and Meister, A. 1974. Effects of amino acids on the function of the isolated perfused rat kidney. Fed. Proc., 33:305.

    Google Scholar 

  • Morgan, H. E., Henderson, M. J., Regen, D. M., and Park, C. R. 1961. Regulation of glucose uptake in muscle. J. Biol. Chem., 236:253.

    PubMed  CAS  Google Scholar 

  • Nishiitsutsuji-Uwo, J. M., Ross, B. D., and Krebs, H. A. 1967. Metabolic activities of the isolated perfused rat kidney. Biochem. J., 103:852.

    PubMed  CAS  Google Scholar 

  • Roobol, A., and Alleyne, G. A. O. 1973. Regulation of renal gluconeogenesis by calcium ions, hormones, and adenosine 3′,5′-cyclic monophosphate. Biochem. J., 134:157.

    PubMed  CAS  Google Scholar 

  • Roobol, A., and Alleyne, G. A. O. 1974. Control of renal cortex ammoniagenesis and its relationship to renal cortical gluconeogenesis. Biochim. Biophys. Acta, 362:83.

    Article  PubMed  CAS  Google Scholar 

  • Ross, B. D. 1972. Perfusion Techniques in Biochemistry, pp. 221–257. Clarenden Press, Oxford.

    Google Scholar 

  • Ross, B. D., Epstein, F. H., and Leaf, A. 1973. Sodium reabsorption in the perfused rat kidney. Am. J. Physiol., 225:1165.

    PubMed  CAS  Google Scholar 

  • Schurek, H. J., Brecht, J. P., Lohfert, H., and Hierholzer, K. 1975. The basic requirements for the function of the isolated cell free perfused rat kidney. Pflügers Arch. Ges. Physiol., 354:349.

    Article  CAS  Google Scholar 

  • Silva, P., Ross, B. D., Charney, A. N., Besarab, A., and Epstein, F. H. 1975. Potassium transport by the isolated perfused rat kidney. J. Clin. Invest., 56:862.

    Article  PubMed  CAS  Google Scholar 

  • Trimble. M. E. 1975. Effects of L-glucose on sodium reabsorption in the isolated perfused rat kidney. Life Sci., 17:1799.

    Article  PubMed  CAS  Google Scholar 

  • Trimble, M. E., and Bowman, R. H. 1973. Renal Na+ and K+ transport: effects of glucose, palmitate, and α-bromopalmitate. Am. J. Physiol., 225:1057.

    PubMed  CAS  Google Scholar 

  • Walter, R., and Bowman, R. H. 1973. Mechanism of inactivation of vasopressin and oxytocin by the isolated perfused rat kidney. Endocrinology, 92:189.

    Article  PubMed  CAS  Google Scholar 

  • Weidemann, M. J., Hems, D. A., and Krebs, H. A. 1969. Effects of added adenine nucleotides on renal carbohydrate metabolism. Biochem. J., 115:1.

    PubMed  CAS  Google Scholar 

  • Weidemann, M. J., and Krebs, H. A. 1969. The fuel of respiration of rat kidney cortex. Biochem. J., 112:149.

    PubMed  CAS  Google Scholar 

  • Welboume, T. C. 1974. Ammonia production and pathways of glutamine metabolism in the isolated perfused rat kidney. Am. J. Physiol., 226:544.

    Google Scholar 

  • Weiss, C., Passow, H., and Rothstein, A. 1959. Autoregulation of flow in isolated rat kidney in the absence of red cells. Am. J. Physiol., 196:1115.

    PubMed  CAS  Google Scholar 

  • Wollenberger, A., Ristau, O., and Schoffa, G. 1960. Eine einfache Technik der extrem Schnellen Abkühlung grosserer Gewebestück. Pflügers Arch. Ges. Physiol., 270:399.

    Article  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Veterans Administration Hospital and Department of Pharmacology, State University of New York Upstate Medical Center, Syracuse, N. Y., USA

    Roger H. Bowman

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  1. Roger H. Bowman
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Editor information

Editors and Affiliations

  1. University of Puerto Rico School of Medicine and Veterans Administration Center, San Juan, Puerto Rico

    Manuel Martinez-Maldonado

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© 1978 Plenum Press, New York

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Bowman, R.H. (1978). Methodology for Study of Isolated Perfused Rat Kidney in Vitro . In: Martinez-Maldonado, M. (eds) Renal Pharmacology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-8894-8_15

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  • DOI: https://doi.org/10.1007/978-1-4615-8894-8_15

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4615-8896-2

  • Online ISBN: 978-1-4615-8894-8

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