Abstract
This study was aimed at determining possible mechanical signal transduction mechanisms in articular cartilage which may be responsible for the control of tissue remodeling. Based on existing data for articular cartilage deformational behavior, the extracellular and pericellular matrices, and the chondrocytes have all been modeled as biphasic materials, with distinct material properties. The cells are embedded and continuously bonded to the surrounding matrix Finite element analysis of the stress, strain, fluid flow, hydraulic pressure and strain energy density were made on a configuration simulating an experiment where a cartilage expiant was loaded in compression. All deformation fields were strongly dependent of chondrocyte material properties relative to the extracellular matrix. The existence of a pericellular matrix seems to reduce the stresses and strains the chondrocyte is subjected to. Confocal microscopy was used to determine the three-dimensional shape and organization of undeformed and deformed chondrocytes from mid-zone cartilage.
Microscopy data indicate that chondrocytes do not deform in an identical manner to the extracellular matrix, thus justifying the distinct material properties assigned to them in the finite element model. This finding led to our proposing an inverse method for determining chondrocyte material properties in situ. This method is based on shape-fitting the observed deformed chondrocyte with the finite element model predictions using an optimization algorithm. Further studies are required to assess the feasibility of this inverse method. Knowledge of the deformational field around the chondrocytes in situ is required for the understanding of mechanical signal transduction mechanisms responsible for mediating the cellular remodeling processes in this tissue.
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© 1993 Birkhäuser Boston
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Mow, V.C., Guilak, F. (1993). Deformation of Chondrocytes within the Extracellular Matrix of Articular Cartilage. In: Bell, E. (eds) Tissue Engineering. Birkhäuser, Boston, MA. https://doi.org/10.1007/978-1-4615-8186-4_13
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DOI: https://doi.org/10.1007/978-1-4615-8186-4_13
Publisher Name: Birkhäuser, Boston, MA
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