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The Role of Ribonucleic Acids in the Organization and Functioning of Ribosomes of E. coli

  • Chapter
Subcellular Biochemistry

Abstract

The study of ribosomal ribonucleic acids (rRNA) has considerably intensified in recent years. The reason for this is not only that rRNA is the major structural component of ribosomal particles, comprising almost two-thirds of their mass and determining the location of the proteins in the ribosome, but also that there is growing evidence for the participation of RNA in the organization of the functional centers of the ribosome. There is scarcely any doubt now that rRNAs are directly involved in the interactions with mRNA, tRNA, and protein-synthesis factors. It has long been believed that these ribosomal centers are composed predominantly of protein subunits and, as a consequence, structural studies on ribosomes have for many years centered on the protein components. As a result, we have a vast number of data on the primary and macromolecular structure of the ribosomal proteins and on their localization in the ribosomal subparticles. But this abundance of data cannot be used immediately for elucidating the structural organization of ribosomes and the mechanism of their functioning, because, until very recently, our knowledge of the structure of RNA in ribosomes has been extremely poor.

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References

  • Araco, A., Belli, M., Giorgi, C., and Onori, G., 1975, The secondary structure of E. coli ribosomes and ribosomal RNA’s: A spectrophotometric approach, Nucl. Acid Res. 2:373–381.

    CAS  Google Scholar 

  • Arnott, S., Fuller, W., Hodgson, A., and Prutton, J., 1968, Molecular conformations and structural transitions of RNA complementary helices and their possible biological significance, Nature (London) 220:561–565.

    CAS  Google Scholar 

  • Aune, K. C., 1977, Molecular interactions between ribosomal proteins. An inspection of S3-S4, S4-S5 and S4-S20 mixtures, Arch. Biochem. Biophys. 180:172–177.

    PubMed  CAS  Google Scholar 

  • Baan, R. A., Duijfjes, J. J., Van Leerdam, E., Van Knippenberg, P. H., and Bosch, L., 1976, Specific in situ cleavage of 16S ribosomal RNA of E. coli interferes with the function of initiation factor IF-1, Proc. Natl. Acad. Sci. U.S.A. 73:702–706.

    PubMed  CAS  Google Scholar 

  • Baan, R. A., Frijmann, M., Van Knippenberg, P. H., and Bosch, L., 1978a, Consequence of a specific cleavage in situ of 16 S ribosomal RNA for polypeptide chain elongation, Eur. J. Biochem. 87:137–142.

    PubMed  CAS  Google Scholar 

  • Baan, R. A., Naaktgeboren, N., Van Charldorp, R., Van Knippenberg, P. H., and Bosch, L., 1978b, Consequence of a specific cleavage in situ of 16S ribosomal RNA for polypeptide chain initiation, Eur. J. Biochem. 87:131–136.

    PubMed  CAS  Google Scholar 

  • Bear, D. G., Schleich, T., Noller, H. F., and Garrett, R. A., 1977, Alteration of 5S RNA conformation by ribosomal proteins L18 and L25, Nucl. Acid Res. 4:2511–2526.

    CAS  Google Scholar 

  • Beaudry, P., Peterson, H. V., Grunberg-Manago, M., and Jacrot, B., 1976, A neutron study of the 30S-ribosome subunit and of the 30S-IF3 complex, Biochem. Biophys. Res. Commun. 72:391–397.

    PubMed  CAS  Google Scholar 

  • Belli, M., Onori, G., Araco, A., and Giorgi, C., 1976, Changes in the ultraviolet absorption of E. coli rRNA on ribosome unfolding, Biopolymers 15:1229–1232.

    PubMed  CAS  Google Scholar 

  • Bochkareva, E. S., Budker, V. G., Girshovich, A. S., Knorre, D. G., and Teplova, N. M., 1973, Specific chemical modification of ribosome in the region of the peptidyl transferase centre, Mol. Biol. 7:278–288.

    CAS  Google Scholar 

  • Bogdanov, A. A., Zimmermann, R. A., Wang, C.-C, and Ford, N. C., Jr., 1978, Conformational changes in 16S ribosomal RNA induced by 30S ribosomal subunit proteins from Escherichia coli, Science 202:999–1001.

    PubMed  CAS  Google Scholar 

  • Branlant, C., Krol, A., Sriwidada, J., and Brimacombe, R., 1976, RNA sequences associated with proteins L1, L9 and L5, L18, L25 in ribonucleoprotein fragments isolated from the 50S subunit of Escherichia coli ribosomes, Eur. J. Biochem. 70:483–492.

    PubMed  CAS  Google Scholar 

  • Branlant, C., Krol, A., Sriwidada, J., and Ebel, J. P., 1977, Characterization of ribonucleoprotein subparticles from 50S ribosomal subunits of Escherichia coli, J. Mol. Biol. 116:443–467.

    PubMed  CAS  Google Scholar 

  • Brimacombe, R., 1978, The structure of the bacterial ribosome, in: Relationship between Structure and Functions of the Prokaryotic Cell, 28th Symposium of the Society of General Microbiology, pp. 1–26, University of Southampton, Cambridge.

    Google Scholar 

  • Brimacombe, R., Nierhaus, K. M., Garrett, R. A., and Wittmann, H. G., 1976, The ribosome of Escherichia coli, in: Progress in Nucleic Acid Research and Molecular Biology (W. E. Cohn, ed.), Vol. 18, pp. 1–44.

    Google Scholar 

  • Brimacombe, R., Stöffler, G., and Wittmann, H. G., 1978, Ribosomes structure, Annu. Rev. Biochem. 47:217–250.

    PubMed  CAS  Google Scholar 

  • Brosius, J., Palmer, M. L., Kennedy, P. J., and Noller, H. F., 1978, Complete nucleotide sequence of 16S ribosomal RNA gene from Escherichia coli, Proc. Natl. Acad. Sci. U.S.A. 75:4801–4805

    PubMed  CAS  Google Scholar 

  • Broude, N. E., Kussova, K. S., Medvedeva, N. L, and Budowsky, E. I., 1978, Initiation factor 3 induced changes in the RNA-protein interactions of E. coli 30S subunit, Bioorgan. Khimiya 4:1687–1689.

    CAS  Google Scholar 

  • Brownlee, G. G., Sanger, F., and Barell, B. G., 1967, Nucelotide sequence of 5S-ribosomal RNA from Escherichia coli, Nature (London) 215:735–737.

    CAS  Google Scholar 

  • Budker, V. G., Girshovich, A. S., and Scobelzina, L. M., 1972, The ability of N-acylaminoacyltRNA, covalently fixed by N-acyl radical on ribosome near the peptidyltransferase centre, for the initiation of polypeptide synthesis, Dokl Akad. Nauk SSSR 207:215–217.

    PubMed  CAS  Google Scholar 

  • Budker, V. G., Girshovich, A. S., Grineva, N. I., Karpova, G. G., Knorre, D. G., and Kobets, N. D., 1973, Specific chemical modification of ribosomes near the mRNA-binding center, Dokl. Akad. Nauk SSSR 211:725–728.

    PubMed  CAS  Google Scholar 

  • Carbon, P., Ehresmann, C., Ehresmann, B., and Ebel, J. P., 1978, The sequence of Escherichia coli ribosomal 16S RNA determined by new rapid gel methods, FEBS Lett. 94:152–156.

    PubMed  CAS  Google Scholar 

  • Carbon, P., Ehresmann, C., Ehresmann, B., and Ebel, J.-P., 1979, The complete nucleotide sequence of the ribosomal 16-S RNA from Escherichia coli. Experimental details and cistron heterogeneities, Eur. J. Biochem. 100:399–410.

    PubMed  CAS  Google Scholar 

  • Changchien, L.-M., and Craven, G., 1976, The function of the N-terminal region of ribosomal protein S4, J. Mol. Biol. 108:381–401.

    PubMed  CAS  Google Scholar 

  • Chapman, N. M., and Noller, H. F., 1977, Protection of specific sites in 16S RNA from chemical modification by association of 30S and 50S ribosomes, J. Mol. Biol. 109:131–149.

    PubMed  CAS  Google Scholar 

  • Chichkova, N. V., Rezapkin, G. V., Teterina, N. L., and Bogdanov, A. A., 1974, RNA-protein interactions in ribosomes. III. Secondary structure of 16 S RNA fragment bound to S4, Mol. Biol. 8:894–899.

    PubMed  CAS  Google Scholar 

  • Cotter, R. I., and Gratzer, W. B., 1969, An infrared study of the conformation of RNA and protein in the ribosome, Eur. J. Biochem. 8:352–356.

    PubMed  CAS  Google Scholar 

  • Cox, R. A., 1966, The secondary structure of ribosomal ribonucleic acid in solution, Biochem. J. 98:841–857.

    PubMed  CAS  Google Scholar 

  • Cundlife, E., 1978, Mechanism of resistance to thiostrepton in the producing organism Streptomyces azureus, Nature (London) 272:792–795

    Google Scholar 

  • Dahlberg, A. E., Tokimatsu, H., Zahalak, M., Reynolds, F., Calvert, P., Rabson, A. B., and Dahlberg, J. E., 1977, Processing of the 17S precursor ribosomal RNA, in: Nucleic Acid-Protein Recognition (H. Vogel, ed.), pp. 509–517, Academic Press, New York.

    Google Scholar 

  • Doty, P., Boedker, H., Fresco, J. R., Haselkorn, R., and Litt, M., 1959, Secondary structure of ribonucleic acids, Proc. Natl. Acad. Sci. U.S.A. 45:482–499.

    PubMed  CAS  Google Scholar 

  • Drigina, E. S., 1975, The CD study of bacterial ribosomes, Ph. D. thesis, Moscow State University.

    Google Scholar 

  • Dunn, J. J., Buzash-Pollert, E., and Studier, F. W., 1978, Mutations of bacteriophage T7 that affect initiation of synthesis of the gene 0.3 protein, Proc. Natl. Acad. Sci. U.S.A. 75:2741–2745.

    PubMed  CAS  Google Scholar 

  • Ehresmann, B., Backendorf, C., Ehresmann, C., and Ebel, J. P., 1977, Characterization of the regions from E. coli 16S RNA covalently linked to ribosomal proteins S4 and S20 after ultraviolet irradiation, FEBS Lett. 78:261–266.

    PubMed  CAS  Google Scholar 

  • Ehresmann, C., Stiegler, P., Carbon, P., and Ebel, J. P., 1977a, Recent progress in the determination of the primary sequence of the 16S RNA of Escherichia coli, FEBS Lett. 84:337–341.

    PubMed  CAS  Google Scholar 

  • Ehresmann, C., Stiegler, P., Carbon, P., Ungewickell, R., and Garrett, R. A., 1977b, A topographical study of the 5′-region of 16S RNA of Escherichia coli in the presence and absence of protein S4, FEBS Lett. 81:188–192.

    PubMed  CAS  Google Scholar 

  • Erdmann, V. A., 1976, Structure and functions of 5S and 5.8S RNA, Prog. Nucl. Acid Res. Mol. Biol. 18:45–90.

    CAS  Google Scholar 

  • Fellner, P., 1974, Structure of the 16S and 23S ribosomal RNAs, in: Ribosomes (M. Nomura, A. Tissières, and P. Lengyel, eds.), pp. 169–191, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.

    Google Scholar 

  • Fox, J. W., Owens, D. P., and Wang, K.-P., 1978, Structural changes of ribosome by the action of ethylene glycol, Biochemistry 17:1357–1364.

    PubMed  CAS  Google Scholar 

  • Garrett, R. A., Ungewickell, E., Newberry, V., Hunter, J., and Wagner, R., 1977, An RNA core in the 30S ribosomal subunit of Escherichia coli and its structural and functional significance, Cell Biol. Int. Rept. 1:487–502.

    CAS  Google Scholar 

  • Gavrilova, L. P., Ivanov, D. A., and Spirin, A. S., 1966, Studies of the structure of ribosomes. III. Stepwise unfolding of the 50S particles without loss of ribosomal proteins, J. Mol. Biol. 16:473–489.

    PubMed  CAS  Google Scholar 

  • Ginzburg, I., and Zamir, A., 1975, Characterization of different conformational forms of 30S ribosomal subunits in isolated and associated states: Possible correlations between structure and function, J. Mol. Biol. 93:465–476.

    PubMed  CAS  Google Scholar 

  • Giri, L., and Franz, A., 1978, The shape of proteins S15 and S18 from the small subunit of the Escherichia coli, ribosomes, FEBS Lett. 87:31–36.

    PubMed  CAS  Google Scholar 

  • Girshovich, A. S., Bochkareva, E. S., Kramorov, V. M., and Ovchinnikov, Yu. A., 1974, E. coli 30S and 50S ribosomal subparticle components in the localization region of the tRNA acceptor terminus, FEBS Lett. 45:213–217.

    PubMed  CAS  Google Scholar 

  • Gualerzi, C., and Pon, C. L., 1973, Nature of the ribosomal binding site for initiation factor 3(IF-3), Biochem. Biophys. Res. Commun. 52:792–799.

    PubMed  CAS  Google Scholar 

  • Heiser, T. L., Davies, J. E., and Dahlberg, J. E., 1972, Mechanism of kasugamycin resistance in Escherichia coli, Nature (London), New Biol. 235:6–9

    Google Scholar 

  • Herr, W., and Noller, H. F., 1979, Protection of specific sites in 23S and 5S RNA from chemical modification by association of 30S and 50S ribosomes, J. Mol. Biol. 130: 421–432

    PubMed  CAS  Google Scholar 

  • Herr, W., Chapman, N. M., and Noller, H., 1979, Mechanism of ribosomal subunit association: discrimination of specific sites in 16S RNA essential for association activity, J. Mol. Biol. 130:433–449.

    PubMed  CAS  Google Scholar 

  • Hochkeppel, H. K., and Craven, G. R., 1976a, Evidence that 16S RNA from E. coli can assume two different biologically active conformations, Nucl. Acid Res. 3:1883–1902.

    CAS  Google Scholar 

  • Hochkeppel, H. K., Spicer, E., and Craven, G. R., 1976, A method of preparing Escherichia coli 16 S RNA possessing previously unobserved 30S ribosomal protein binding sites, J. Mol. Biol. 101:155–170.

    PubMed  CAS  Google Scholar 

  • Isaacs, S. T., Shen, C. K. J., Hearst, J. E., and Rapoport, H., 1977, Synthesis and characterization of new psoralen derivatives with superior photoreactivity with DNA and RNA, Biochemistry 16:1058–1064.

    PubMed  CAS  Google Scholar 

  • Kim, S. H., Suddath, F. L., Quigley, G. J., McPherson, A., Sussman, J. L., Wang, A. H. J., Seeman, W. C., and Rich, A., 1974, Three-dimensional tertiary structure of yeast phenylalanine transfer RNA, Science 185:435–440.

    PubMed  CAS  Google Scholar 

  • Kopylov, A. M., Shalaeva, E. S., and Bogdanov, A. A., 1974, Structural transformations of ribonucleoprotein particles—intermediate products of 30S ribosomal subunits self-assembly, Dokl. Akad. Nauk SSSR 216:1178–1181.

    PubMed  CAS  Google Scholar 

  • Kopylov, A. M., Chichkova, N. V., Bogdanov, A. A., and Vasilenko, S. K., 1975, Complementary binding of oligonucleotides with 16S RNA and ribosomal ribonucleoproteins, Mol. Biol. Rep. 2:95–100.

    PubMed  CAS  Google Scholar 

  • Kopylov, A. M., Teterina, N. L., Potapov, A. P., Vasilenko, S. K., and Bogdanov, A. A., 1976, RNA-protein interactions in ribosomes. V. Accessibility of single-and double-stranded regions of RNA in bacterial ribosomal particles with different protein composition, Bioorg. Khim. 2:1642

    CAS  Google Scholar 

  • Koteliansky, V. E., Domogatsky, S. P., Gudkov, A. T., and Spirin, A. S., 1977, Elongation factor dependent reactions of ribosomes deprived of proteins L7 and L12, FEBS Lett. 73:6–11.

    PubMed  CAS  Google Scholar 

  • Kühlbrandt, W., and Garrett, A., 1978, A ribonucleoprotein core in the 50S ribosomal subunit of Escherichia coli, FEBS Lett. 94:207–211

    PubMed  Google Scholar 

  • Kurland, C. G., 1974, Functional organization of the 30 S ribosomal subunit, in: Ribosomes (N. Nomura, A. Tissières, and P. Lengyel, eds.), pp. 309–331, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.

    Google Scholar 

  • Kurland, C. G., 1977a, Structure and function of the bacterial ribosome, Annu. Rev. Biochem. 46:173–200.

    PubMed  CAS  Google Scholar 

  • Kurland, C. G., 1977b, Aspects of ribosome structure and function, in: Molecular Mechanisms of Protein Biosynthesis (H. Weissbach and S. Pestka, eds.), pp. 81–113, Academic Press, New York.

    Google Scholar 

  • Lai, C., Dahlberg, J. E., and Weisblum, B., 1973a, Structure of an inducibly methylatable nucleotide sequence in 23S RNA from erythromycin-resistant Staphylococcus aureus, Biochemistry 12:457–460.

    PubMed  CAS  Google Scholar 

  • Lai, C., Weisblum, B., Fahnestock, S. R., and Nomura, M., 1973b, Alteration of 23S ribosomal RNA and etythromycin-induced resistance to lincomycin and spiramycin in Staphylococcus aureus, J. Mol Biol. 74:67–72.

    PubMed  CAS  Google Scholar 

  • Lake, J. A., 1976, Ribosome structure determined by electron microscopy of Escherichia coli small subunits and monomeric ribosomes, J. Mol. Biol. 105:131–159.

    PubMed  CAS  Google Scholar 

  • Lake, J. A., and Kahan, L., 1975, Ribosomal proteins S5, S1 1, S13, S19 localized by electron microscopy of antibody-labeled subunits, J. Mol. Biol. 99:631–644.

    PubMed  CAS  Google Scholar 

  • Laughrea, M., Dondon, J., and Grunberg-Manago, M., 1978, The relationship between the 3′-end of 16S RNA and the binding of initiation factor IF-3 to the 30S subunit of E. coli, FEBS Lett. 91:265–268.

    PubMed  CAS  Google Scholar 

  • Lemieux, G., Lefevre, J.-F., and Daune, M., 1974, Effect of reconstitution conditions on the structure of Escherichia coli 30S ribosomal-subunit components, Eur. J. Biochem. 49:185–194.

    PubMed  CAS  Google Scholar 

  • Lerman, M. I., Spirin, A. S., Gavrilova, L. P., and Golov, V. F., 1966, Studies on the structure of ribosomes. II. Stepwise dissociation of protein from ribosomes by caesium chloride and reassembly of ribosome-like particles, J. Mol. Biol. 15:268–281.

    PubMed  CAS  Google Scholar 

  • Lim, V. I., Mazanov, A. L., and Efimov, A. V., 1978, Structure of ribosomal proteins. Prediction of tertiary structure of proteins from small 30S subunit of Escherichia coli ribosomes, Dokl. Akad. Nauk SSSR 242:1219–1222.

    PubMed  CAS  Google Scholar 

  • Mackie, G. A., and Zimmermann, R. A., 1978, RNA-protein interactions in the ribosome. IV. Structure and properties of binding sites for proteins S4, S16/17 and S20 in the 16S RNA, J. Mol.Biol. 121:17–39.

    PubMed  CAS  Google Scholar 

  • Martinez, O., Vazquez, D., and Modolell, J., 1978, Streptomycin and viomycin-induced conformational changes of ribosomes detected by iodination, FEBS Lett. 87:21–25.

    PubMed  CAS  Google Scholar 

  • Miall, S., and Walker, I., 1969, Structural studies on ribosomes. II. Denaturation and sedimentation of ribosomal subunits unfolded in EDTA, Biochim. Biophys. Acta 174:551–562.

    PubMed  CAS  Google Scholar 

  • Michalski, C. J., Sells, B. H., and Wahba, A. J., 1976, Molecular morphology of ribosomes: effect of chain initiation factor 3 on 30S subunit conformation, FEBS Lett. 71:347–350.

    PubMed  CAS  Google Scholar 

  • Michalski, C. J., Sells, B. H., Chowdhury, R. M., and Wahba, A. J., 1978, Molecular morphology of ribosomes. Influence of chain-initiation factor IF-3 and ribosomal protein S1 on ribosome conformation, Eur. J. Biochem. 89:589–594.

    PubMed  CAS  Google Scholar 

  • Mikhailova, I. M., and Bogdanov, A. A., 1970, Electrophoresis of ribosomes and ribosomal RNA in polyacrylamide gel, Biokhimiya 35:403–408.

    CAS  Google Scholar 

  • Möller, K., Rinke, J., Ross, A., Buddie, G., and Brimacombe, R., 1977, The use of formaldehyde in RNA-protein crosslinking studies with ribosomal subunits of E. coli, Eur. J. Biochem. 76:175–187.

    PubMed  Google Scholar 

  • Möller, K., Zwieb, C., and Brimacombe, R., 1978, Identification of the oligonucleotide and oligopeptide involved in an RNA-protein crosslink induced by ultraviolet irradiation of Escherichia coli 30S ribosomal subunits, J. Mol. Biol. 126:489–506.

    PubMed  Google Scholar 

  • Muto, A., and Zimmermann, R. A., 1978, Conformation and stability of ribosomal protein binding sites in the 16S RNA, J. Mol. Biol. 121:1–15.

    PubMed  CAS  Google Scholar 

  • Muto, A., Ehresmann, C., Fellner, P., and Zimmermann, R. A., 1974, RNA-protein interactions in the ribosome. I. Characterization and ribonuclease-digestion of 16S RNA-ribosomal protein complexes, J. Mol. Biol. 86:411–432.

    PubMed  CAS  Google Scholar 

  • Noller, H. F., and Herr, W., 1974, Accessibility of 5S RNA in 50S ribosomal subunits, J. Mol. Biol. 90: 181–184.

    PubMed  CAS  Google Scholar 

  • Nomura, M., and Held, W. A., 1974, Reconstitution of ribosomes: Studies of ribosome structure, function and assembly, in: Ribosomes (M. Nomura, A. Tissières, P. Lengyel, eds.), pp. 192–224, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.

    Google Scholar 

  • Nomura, M., Sidikaro, J., Jakes, K., and Zinder, N., 1974, Effect of colicin E3 on bacterial ribosome, in: Ribosomes (M. Nomura, A. Tissières, P. Lengyel, eds.), Part II, pp. 805–814, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.

    Google Scholar 

  • Ofengand, J., 1977, tRNA and aminoacyl-tRNA synthetase, in: Molecular Mechanisms of Protein Biosynthesis (H. Weissbach and S. Pestka, eds.), pp. 8–69, Academic Press, New York.

    Google Scholar 

  • Ofengand, J., and Henes, C., 1969, The function of pseudouridylic acid in transfer ribonucleic acid. II. Inhibition of amino acyl transfer ribonucleic acid-ribosome complex formation by ribothymidylyl-pseudouridylyl-cytidylyl-guanosine 3′-phosphate, J. Biol. Chem. 244: 6241–6253.

    PubMed  CAS  Google Scholar 

  • Ofengand, J., Liou, R., Kohat, J., III, Schwartz, I., and Zimmermann, R. A., 1979, Covalent cross-linking of transfer ribonucleic acid to the ribosomal P site. Mechanism and site of reaction in transfer ribonucleic acid, Biochemistry 18:4322–4332.

    PubMed  CAS  Google Scholar 

  • Österberg, R., Sjöberg, B., Garrett, R. A., and Littlechild, J., 1977, Small-angle X-ray scattering study of the 16S binding protein S4 from Escherichia coli ribosomes, FEBS Lett. 73:25–28.

    PubMed  Google Scholar 

  • Paradies, H. H., and Franz, A., 1976, Geometry of the protein S4 from Escherichia coli ribosomes, Eur. J. Biochem. 67:23–29.

    PubMed  CAS  Google Scholar 

  • Pelligrini, M., and Cantor, C. R., 1977, Affinity labelling of ribosomes, in: Molecular Mechanisms of Protein Biosynthesis (H. Weissbach and S. Pestka, eds.), pp. 203–241, Academic Press, New York.

    Google Scholar 

  • Politz, S. M., and Glitz, D. G., 1977, Localization of N6-dimethyladenosine in the small subunit of E. coli ribosomes by electron microscopy of an antibody-ribosome complex, Proc. Natl. Acad. Sci. U.S.A. 74:1468–1472.

    PubMed  CAS  Google Scholar 

  • Pon, C. L., and Gualerzi, C., 1976, The role of 16S RNA in ribosomal binding of IF-3, Biochemistry 15:804–811.

    PubMed  CAS  Google Scholar 

  • Potapov, A. P., and Bogdanov, A. A., 1974, The model of spatial organization of RNA and proteins in ribosomes, Mol. Biol. 8:425–432.

    CAS  Google Scholar 

  • Potapov, A. P., and Bogdanov, A. A., 1977, Compact structure of the small ribosomal subunit and its RNAs studied by fluorescence spectroscopy and sedimentation analysis, Mol. Biol. 11:545–554.

    CAS  Google Scholar 

  • Potapov, A. P., Shalaeva, E. S., Borisova, O. F., and Bogdanov, A. A., 1973, Isotherms of absorption of acriflavine on 16S RNA and ribonucleoprotein particles obtained from ribosomes of Escherichia coli, Dokl. Akad. Nauk SSSR 208:468–471

    PubMed  CAS  Google Scholar 

  • Prince, J. B., Hixson, S. S., and Zimmermann, R. A., 1979, Photochemical cross-linking of tRNALys and tRNA2 Glu to 16S RNA at the P site of Escherichia coli ribosomes, J. Biol. Chem. 254:4745–4749.

    PubMed  CAS  Google Scholar 

  • Rezapkin, G. V., Shatsky, I. N., and Bogdanov, A. A., 1977, Use of acrolein and acrylamide copolymers for investigation of internal organization of small ribosomal subunits, Bioorg. Khim. 3:348–355.

    CAS  Google Scholar 

  • Richter, D., Erdmann, V. A., and Sprinzl, M., 1973, Specific recognition of GTøC loop (loop IV) of tRNA by 50S ribosomal subunits from E. coli, Nature (London), New Biol. 246:132–135.

    CAS  Google Scholar 

  • Robertus, J. D., Ladner, J. E., Finch, J. T., Rhodes, D., Brown, R. S., Clark, B. F. C., and Klug, A., 1974, Structure of yeast phenylalanine tRNA at 3Ã… resolution, Nature (London) 250:546.

    CAS  Google Scholar 

  • Rohde, M. F., O’Brien, S., Cooper, S., and Aune, K. C., 1975, Physical properties of some ribosomal proteins in solution and evidence for molecular interactions between isolated ribosomal proteins, Biochemistry 14:1079–1087.

    PubMed  CAS  Google Scholar 

  • Ross, A., and Brimacombe, R., 1979, Experimental determination of interacting sequences in ribosomal RNA, Nature (London) 281:271–276.

    CAS  Google Scholar 

  • Sander, G., Parlato, G., Grechet, J. B., Nagel, K., and Parmeggiani, A., 1978, Regulation of turnover GTPase activity of elongation factor G: The 30S-coupled and 30S-uncoupled reactions. Coordinated effects of cations, pH and polyamines, Eur. J. Biochem. 86:555–563.

    PubMed  CAS  Google Scholar 

  • Santer, M., and Santer, U., 1972, Characterization of the 5′ and 3′ ends of the 16S ribonucleic acid from T,-ribonuclease-treated 30S ribosomes, Biochemistry 11:786–791.

    PubMed  CAS  Google Scholar 

  • Santer, M., and Shane, S., 1977, Area of 16S ribonucleic acid at or near the interface between 30S and 50S ribosomes of Escherichia coli, J. Bacteriol. 130:900–910.

    PubMed  CAS  Google Scholar 

  • Schulte, C., Morrison, C. A., and Garrett, R. A., 1974, Protein-ribonucleic acid interaction in Escherichia coli ribosomes. Solution studies on S4-S16 ribonucleic acid and L24-23S ribonucleic acid binding, Biochemistry 13:1032–1037.

    PubMed  CAS  Google Scholar 

  • Schwartz, U., Möller, A., and Gassen, H. G., 1978, Induced structural transitions in tRNA, FEBS Lett. 90:189

    Google Scholar 

  • Seals, A. A., and Champrey, W. S., 1976, Conformational changes in Escherichia coli ribosomal ribonucleic acid, Biochem. Biophys. Res. Commun. 72:753–760.

    PubMed  CAS  Google Scholar 

  • Serdyuk, I. N., 1978, A method of joint use of electromagnetic and neutron scattering: A study of internal ribosomal structure, Meth. Enzymol. 59:750–775.

    Google Scholar 

  • Serdyuk, I. N., and Grenader, A. K., 1975, Joint use of light, X-ray and neutron scattering for investigation of RNA and protein mutual distribution within the 50S subparticle of E. coli ribosomes, FEBS Lett. 59:133–136.

    PubMed  CAS  Google Scholar 

  • Serdyuk, I. N., Smirnov, N. G., Ptitsyn, O. B., and Fedorov, B. A., 1970, On the presence of a dense internal region in the 50S subparticle of E. coli ribosomes, FEBS Lett. 9:324–326.

    PubMed  CAS  Google Scholar 

  • Serdyuk, I. N., Zaccai, G., and Spirin, A. S., 1978, Globular conformation of some ribosomal proteins in solution, FEBS Lett. 94:349–352.

    PubMed  CAS  Google Scholar 

  • Shatsky, I. N., Boni, I. V., Chichkova, N. V., and Bogdanov, A. A., 1971a, RNA-protein interactions in the ribosomes. Circular dichroism of 30S ribosomal subunits from Escherichia coli and its components, Mol. Biol. 5:817–825.

    Google Scholar 

  • Shatsky, I. N., Chichkova, N. V., and Bogdanov, A. A., 1971b, RNA-protein interaction in the ribosomes. Thermal denaturation of RNA in the ribosomes, Mol. Biol. 5:149–155.

    Google Scholar 

  • Shatsky, I. N., Mochalova, L. V., and Bogdanov, A. A., 1978, Modification of RNA 3′-terminal nucleosides by lactose-containing haptenes, Bioorg. Khim. 4:418–420.

    Google Scholar 

  • Shatsky, I. N., Mochalova, L. V., Kojouharova, M., Bogdanov, A. A., and Vasiliev, V. D., 1979, Localization of the 3′-end of E. coli ribosomal 16S RNA by electron microscopy of antibody-labelled subunits, J. Mol. Biol. 133:501–515.

    PubMed  CAS  Google Scholar 

  • Shen, C.-K. J., and Hearst, J. R., 1976, Psoralen-cross-linked secondary structure map of singlestranded virus DNA, Proc. Natl. Acad. Sci. U.S.A. 73:2649–2653.

    PubMed  CAS  Google Scholar 

  • Shine, J., and Dalgarno, L., 1975, Determinant of cistron specificity in bacterial ribosomes, Nature (London) 254:34–38.

    CAS  Google Scholar 

  • Skripkin, E. A., Vinogradov, S., Kopylov, A. M., Berlin, Yu., and Bogdanov, A. A., 1979, rRNA topography in the ribosome IV the accessibility of the 5′ end region of 16S rRNA, Mol. Biol. Rep. 5:221–224.

    PubMed  CAS  Google Scholar 

  • Sloof, P., Hunter, J. B., Garrett, R. A., and Branlant, C., 1978, RNA-RNA interactions in the binding site of protein L24 on 23S ribosomal RNA of Escherichia coli. 1. Evidence for their occurrence between widely separated sequence regions, Nucl. Acid Res. 5:3503–3513.

    CAS  Google Scholar 

  • Spierer, P., Bogdanov, A. A., and Zimmermann, R. A., 1978, Parameters for the interaction of ribosomal proteins L5, L18 and L25 with 5S RNA from E. coli, Biochemistry 17:5394–5398.

    PubMed  CAS  Google Scholar 

  • Spirin, A. S., 1964, Macromolecular Structure of Ribonucleic Acids, Reinhold, New York.

    Google Scholar 

  • Spirin, A. S., 1974, Structural transformations of ribosomes (dissociation, unfolding and disassembly), FEBS Lett. 40(Suppl.):538–546.

    Google Scholar 

  • Spirin, A. S., and Gavrilova, L. P., 1971, Ribosoma, Nauka, Moscow.

    Google Scholar 

  • Spirin, A. S., Gavrilova, L. P., Bresler, S. E., and Mosevitsky, M. I., 1959, Studies on macromolecular structure of RNA from TMV, Biokhimiya 24:938–947.

    CAS  Google Scholar 

  • Spirin, A. S., Serdyuk, I. N., Vasiliev, V. D., and Lim, V. I., 1979, Principles of structural organization of ribosomes, in: Proceedings of the 12th FEBS Meeting, Dresden, 1978 (Gene Functions), (S. Rosenthal et al., eds.), Vol. 51, pp. 365–377, Pergamon Press, Oxford and N.Y.

    Google Scholar 

  • Spitnik-Elson, P., and Elson, D., 1976, Studies on the ribosomes and its components, Prog. Nucl. Acid Res. Mol. Biol. 17:77–98.

    CAS  Google Scholar 

  • Spitnik-Elson, P., Elson, D., Abramowitz, R., and Avital, S., 1978, A large nucleoprotein fragment of the 50S ribosomal subunit of Escherichia coli, Biochim. Biophys. Acta 521:308–323

    PubMed  CAS  Google Scholar 

  • Steitz, J., and Jakes, K., 1975, How ribosomes select initiator regions in mRNA: Base pair formation between the 3′ terminus of 16S rRNA and the mRNA during initiation of protein synthesis of E. coli, Proc. Natl. Acad. Sci. U.S.A. 72:4734–4738.

    PubMed  CAS  Google Scholar 

  • Stöffler, G., and Wittmann, H. G., 1977, Primary structure and three-dimensional arrangement of proteins within the E. coli ribosome, in: Molecular Mechanisms of Protein Biosynthesis (H. Weissbach and S. Pestka, eds.), pp. 117–202, Academic Press, New York.

    Google Scholar 

  • Stuhrmann, H. B., Haas, J., Ibel, K., De Wolf, B., Koch, M. H. J., Parfait, R., and Crichton, R. R., 1976, New low resolution model for 50S subunit of E. coli ribosomes, Proc. Natl. Acad. Sci. U.S.A. 73:2379–2383.

    PubMed  CAS  Google Scholar 

  • Taniguchi, T., and Weissmann, C., 1978, Inhibition of Qβ RNA 70S ribosome initiation complex formation by an oligonucleotide complementary to the 3′ terminal region of E. coli 16 S ribosomal RNA, Nature (London) 275:770–772.

    CAS  Google Scholar 

  • Teterina, N. L., Kagramanova, V. K., Kopylov, A. M., and Bogdanov, A. A., 1978a, Topography of rRNA in ribosomes. II. Nonanucleotide released from 30S subunits of E. coli ribosomes with RNase, Bioorg. Khim. 4:415–417.

    CAS  Google Scholar 

  • Teterina, N. L., Kopylov, A. M., and Bogdanov, A. A., 1978b, Topography of rRNA in ribosomes. I. Effect of pancreatic RNase on 30S subunits of E. coli ribosomes, Biokhimiya 43:229.

    CAS  Google Scholar 

  • Thammana, P., and Cantor, C. R., 1978, Studies on ribosome structure and interactions near the m6 2A m6 2A sequence, Nucl. Acid Res. 5:805–823.

    CAS  Google Scholar 

  • Thammana, P., and Held, W. A., 1974, Methylation of 16S RNA during ribosome assembly in vitro, Nature (London) 251:682–688

    CAS  Google Scholar 

  • Thammana, P., Cantor, C. R., Wollenzien, P., and Hearst, J. E., 1979, Cross-linking studies on the organization of the 16S ribosomal RNA within the 30S Escherichia coli ribosomal subunits, J. Mol. Biol. 135:271–284.

    PubMed  CAS  Google Scholar 

  • Thomas, G., Sweeney, R., Chang, C., and Noller, H., 1975, Identification of protein functionally altered by chemical modification of the transfer RNA and polyuridilic binding sites of 30 S ribosomal subunits, J. Mol. Biol. 95:91–102.

    PubMed  CAS  Google Scholar 

  • Thurlow, D. L., and Zimmermann, R. A., 1978, Conservation of ribosomal protein binding sites in prokaryotic 16S RNAs, Proc. Natl. Acad. Sci. U.S.A. 75:2859–2863.

    PubMed  CAS  Google Scholar 

  • Tischendorf, G. W., Zeichhardt, H., and Stöffler, G., 1975, Architecture of the E. coli ribosomes as determined by immune electron microscopy, Proc. Natl. Acad. Sci. U.S.A. 72:4820–4824.

    PubMed  CAS  Google Scholar 

  • Towbin, H., and Elson, D., 1978, A photoaffinity labelling study of the messenger RNA-binding region of E. coli ribosomes, Nucl. Acid Res. 5:3389–3407.

    CAS  Google Scholar 

  • Tritton, T. R., and Crothers, D. M., 1976, Physical characterization of ribosomal nucleoprotein complex, Biochemistry 15:4377–4385.

    PubMed  CAS  Google Scholar 

  • Ulmer, E., Meinke, M., Ross, A., Fink, G., and Brimacombe, R., 1978, Chemical cross-linking of protein to RNA within intact ribosomal subunits from E. coli, Mol. Gen. Genet. 160:183–193

    PubMed  CAS  Google Scholar 

  • Ungewickell, E., Garrett, R. A., and Le Bret, M., 1977a, Native and denatured structures within the 5′-region of 16S ribosomal RNA from Escherichia coli, FEBS Lett. 84:37–42.

    PubMed  CAS  Google Scholar 

  • Ungewickell, E., Garrett, R. A., Ehresmann, C., Stiegler, P., and Carbon, P., 1977b, Further characterization of the RNA structure in the binding region of protein S4 on 16S ribosomal RNA of E. coli, FEBS Lett. 81:193–198.

    PubMed  CAS  Google Scholar 

  • Van Duin, J., Kurland, C. G., Dondon, J., and Grunberg-Manago, M., 1975, Near neighbours of IF-3 bound to 30S ribosomal subunits, FEBS Lett. 59:287–290.

    PubMed  Google Scholar 

  • Van Duin, J., Kurland, C. G., Dondon, J., Grunberg-Manago, M., Branlant, C., and Ebel, J. P., 1976, New aspects of the IF3-ribosome interaction, FEBS Lett. 62:111–114.

    PubMed  Google Scholar 

  • Van Knippenberg, P. H., 1975, A possible role of the 5′-terminal sequence of 16S ribosomal RNA in the recognition of initiation sequences for protein synthesis, Nucl. Acid Res. 2:79–85.

    Google Scholar 

  • Vasiliev, V. D., 1974, Morphology of the ribosomal 30S subparticle according to electron microscopic data, Acta Biol. Med. Germ. 33:779–793.

    PubMed  CAS  Google Scholar 

  • Vasiliev, V. D., Koteliansky, V. E., and Rezapkin, G. V., 1977a, The complex of 16S RNA with proteins S4, S7, S8, S15 retains the main morphological features of the 30S ribosomal subparticle, FEBS Lett. 79:170.

    PubMed  CAS  Google Scholar 

  • Vasiliev, V. D., Koteliansky, V. E., Shatsky, I. N., and Rezapkin, G. V., 1977b, Structure of the ribosomal 16 RNA-protein S4 complex as revealed by electron microscopy, FEBS Lett. 84:43–47.

    PubMed  CAS  Google Scholar 

  • Vasiliev, V. D., Seiivanova, O. M., and Koteliansky, V. E., 1978, Specific self-packing of the ribosomal 16S RNA, FEBS Lett. 95:273–276.

    PubMed  CAS  Google Scholar 

  • Wagner, R., and Garrett, R. A., 1978, A new RNA-RNA cross-linking reagent and its application to ribosomal 5S RNA, Nucl. Acid Res. 5:4065–4075.

    CAS  Google Scholar 

  • Wagner, R., Gassen, H. G., Ehesmann, C., Stiegler, P., and Ebel, J.-P., 1976, Identification of a 16 S RNA sequence located in the coding site of 30 S ribosomes, FEBS Lett. 67:312–315.

    PubMed  CAS  Google Scholar 

  • Wollenzien, P., Hearst, J. E., Thammana, P., and Cantor, C. R., 1979, Base pairing between distant regions of the Escherichia coli, 16S RNA in solution, J. Mol. Biol. 135:255–270.

    PubMed  CAS  Google Scholar 

  • Yu, R. S. T., and Wittmann, H. G., 1973, The sequence of steps in the attachment of 5S RNA to cores of E. coli ribosomes, Biochim. Biophys. Acta 324:375–385.

    PubMed  CAS  Google Scholar 

  • Zimmermann, R. A., 1974, Protein-RNA interactions in the ribosome, in: Ribosomes (M. Nomura, A. Tissières, and P. Lengyel, eds.), pp. 225–270, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.

    Google Scholar 

  • Zimmermann, R. A., Mackie, G. A., Muto, A., Garrett, R. A., Ungewickell, E., Ehresmann, C., Stiegler, P., Ebel, J. P., and Fellner, P., 1975, Location and characterization of ribosomal protein binding sites in the 16S RNA of E. coli, Nucl. Acid Res. 2:279–302.

    CAS  Google Scholar 

  • Zimmermann, R. A., Gates, S. M., Schwartz, I., and Ofengand, J., 1979, Covalent crosslinking of transfer ribonucleic acid to the ribosomal P site. Site of reaction in 16S ribonucleic acid, Biochemistry, 18:4333–4339.

    PubMed  CAS  Google Scholar 

  • Zwieb, C., and Branlant, R., 1979, RNA-protein cross-linking in Escherichia coli 30S ribosomal subunits: Precise localization of the nucleotide in 16S RNA which is coupled to protein S7 by ultraviolet irradiation, Nucl. Acid Res. 6:1775–1790.

    CAS  Google Scholar 

  • Zwieb, C., and Brimacombe, R., 1978, Evidence for RNA-RNA cross-link formation in E. coli ribosomes, Nucl. Acid Res. 5:1189–1206.

    CAS  Google Scholar 

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Authors and Affiliations

  1. A. N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Moscow, 117234, U.S.S.R.

    A. A. Bogdanov, A. M. Kopylov & I. N. Shatsky

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  1. A. A. Bogdanov
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  2. A. M. Kopylov
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  3. I. N. Shatsky
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  1. University College London, London, England

    Donald B. Roodyn

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Bogdanov, A.A., Kopylov, A.M., Shatsky, I.N. (1980). The Role of Ribonucleic Acids in the Organization and Functioning of Ribosomes of E. coli . In: Roodyn, D.B. (eds) Subcellular Biochemistry. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7948-9_2

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