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Aqueous size exclusion chromatography

  • Chapter
Size Exclusion Chromatography

Abstract

Aqueous SEC was first reported in 1959 by Porath and Flodin [1]. They separated proteins and salts according to molecular size by using cross-linked dextran gels. Since then it has been widely employed, especially in the field of biochemistry, for various purposes such as purification of proteins and nucleic acids, estimation of molecular masses of proteins and determination of molecular mass distributions of polysaccharides. In addition, it has been a powerful tool for the determination of molecular mass distributions of watersoluble synthetic polymers since high-performance aqueous SEC was realized in 1978 by the development of semirigid microparticulate macroporous supports based on hydrophilic synthetic polymers [2–4].

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Authors
  1. Y. Kato
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Editor information

Editors and Affiliations

  1. Polymer Research Group, Department of Chemistry, University of Lancaster, LA1 4YA, Lancaster, UK

    B. J. Hunt

  2. RAPRA Technology Ltd, SY4 4NR, Shawbury, Shrewsbury, UK

    S. R. Holding

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© 1989 Springer Science+Business Media Dordrecht

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Kato, Y. (1989). Aqueous size exclusion chromatography. In: Hunt, B.J., Holding, S.R. (eds) Size Exclusion Chromatography. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7861-1_7

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  • DOI: https://doi.org/10.1007/978-1-4615-7861-1_7

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4615-7863-5

  • Online ISBN: 978-1-4615-7861-1

  • eBook Packages: Springer Book Archive

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