Abstract
The activities of the enzymes which catalyse the last two steps of the pyrimidine de novo synthetic pathway — orotic acid phosphoribosyltransferase (OPRT) and orotidine-5′-monophosphate decarboxylase (ODC) — are contained in a single bifunctional protein, UMP synthase (UMPS) (1). Patients with the autosomal recessive disorder, hereditary oroticaciduria, have coordinate deficiencies of both these activities (2). Previous assays for the human enzyme have measured CO2 release from [14C]-orotic acid (2). This paper reports results using an HPLC method employing an ion-pair reversed phase system, developed to analyse the products of UMPS activity in lysates of RBC and CVS.
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References
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© 1991 Plenum Press, New York
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Fairbanks, L.D., Duley, J.A., Shores, A.J., Simmonds, H.A. (1991). HPLC Assay of Uridine Monophosphate Synthase (UMPS) in Chorionic Villus Samples (CVS) and Erythrocytes (RBC). In: Harkness, R.A., Elion, G.B., Zöllner, N. (eds) Purine and Pyrimidine Metabolism in Man VII. Advances in Experimental Medicine and Biology, vol 309B. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-7703-4_8
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DOI: https://doi.org/10.1007/978-1-4615-7703-4_8
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