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Automated Quantitative Analysis for Orotidine and Uridine/Thymine in Urine by High-Performance Liquid Chromatography with Column Switching

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Purine and Pyrimidine Metabolism in Man VII

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 309B))

Abstract

The measurement of urinary orotidine, uridine and thymine which are intermediates in pyrimidine biosynthesis, is important for screening and diag-nosis inborn ergors of metabolism, such as urea cyclel and pyrimidine metabo-lism disorders 2 , as well as the measurement of urinary orotic acid and uracil l,3. Several high-performance liquid chromatography (HPLC) methods have been reported by a number of researchers. However, these HPLC methods for uridine and thymine analysis require rather complicated sample preparation in order to obtain accurate results 1. Although, a simple method for measuring orotidine and orotic acid were recently reported 4, it is not capable of evaluating near-normal levels of orotidine.

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References

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Author information

Authors and Affiliations

  1. Department of Pediatrics, Nagoya City Higashi General Hospital, 1-2-23 Wakamizu, Chikusa-ku, Nagoya 464, Japan

    Kiyoshi Kidouchi, Chie Nakamura & Toshiyuki Katoh

  2. Department of Pediatrics, Nagoya City University Medical School, 1 Kawasumi, Mizuho-ku, Nagoya 467, Japan

    Tetsuya Kibe, Satoru Ohba & Yoshiro Wada

Authors
  1. Kiyoshi Kidouchi
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  2. Chie Nakamura
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  3. Toshiyuki Katoh
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  4. Tetsuya Kibe
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  5. Satoru Ohba
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  6. Yoshiro Wada
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Editor information

Editors and Affiliations

  1. Institute of Child Health, London, UK

    R. Angus Harkness

  2. Burroughs Wellcome Co., Research Triangle Park, North Carolina, USA

    Gertrude B. Elion

  3. Universität München, München, Germany

    Nepomuk Zöllner

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© 1991 Plenum Press, New York

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Kidouchi, K., Nakamura, C., Katoh, T., Kibe, T., Ohba, S., Wada, Y. (1991). Automated Quantitative Analysis for Orotidine and Uridine/Thymine in Urine by High-Performance Liquid Chromatography with Column Switching. In: Harkness, R.A., Elion, G.B., Zöllner, N. (eds) Purine and Pyrimidine Metabolism in Man VII. Advances in Experimental Medicine and Biology, vol 309B. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-7703-4_7

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  • DOI: https://doi.org/10.1007/978-1-4615-7703-4_7

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4615-7705-8

  • Online ISBN: 978-1-4615-7703-4

  • eBook Packages: Springer Book Archive

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