Abstract
Phase-sensitive detection of fluorescence (PSDF) is a newly developed method with numerous potential applications in physical, chemical, and biological research. This method is based upon the principles discussed in Chapter 3 with regard to the measurement of fluorescence lifetimes by the phase shift and demodulation methods. It is convenient to discuss this topic separately, with emphasis on the analysis of samples which display ground state heterogeneity. In a subsequent chapter we will describe the application of this technique to excited state reactions.
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References
Lakowicz, J. R., and Cherek, H., 1981, Phase-sensitive fluorescence spectroscopy. A new method to resolve fluorescence lifetimes or emission spectra of components in a mixture of fluorophores, J. Biochem. Biophys. Methods 5:19–35.
Lakowicz, J. R., and Cherek, H., 1981, Resolution of heterogeneous fluorescence from proteins and aromatic amino acids by phase-sensitive detection of fluorescence,J. Biol. Chem. 256:6348–6353.
Knutson, J. R., Walbridge, D. G., and Brand, L., 1982, Decay-associated fluorescence spectra and the heterogeneous emission of alcohol dehydrogenase,Biochemistry (in press).
Haar, H. P., and Hauser, M., 1978,Rev. Sci. Instrum. 49:632–633.
Badea, M. G., and Brand, L., 1979, Time-resolved fluorescence measurements, Methods Enzymol. 61:378–425.
Meech, S. R., O’Connor, D. V., Roberts, A. J., and Phillips, D., 1981, On the construction of nanosecond time-resolved emission spectra, Photochem. Photobiol. 38:159–172.
Lakowicz, J. R., and Cherek, H., 1980, Dipolar relaxation in proteins on the nanosecond timescale observed by wavelength-resolved phase fluorometry of tryptophan fluorescence, J. Biol. Chem. 255:831–834.
Ross, J. B. A., Schmidt, C. J., and Brand, L., 1981, Time-resolved fluorescence of the two tryptophans in horse liver alcohol dehydrogenase, Biochemistry 20:4369–4377.
Grinvald, A., and Steinberg, J. Z., 1976, The fluorescence decay of tryptophan residues in native and denatured proteins,Biochem. Biophys. Acta 427:663–678.
Rayner, D. M., and Szabo, A., 1978, Time-resolved fluorescence of aqueous tryptophan, Can. J. Chem. 56:743–745.
Lakowicz, J. R., and Keatings, S., 1983, Binding of an indole derivative to micelles as quantified by phase sensitive detection of fluorescence, J. Biol Chem. (1981).
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© 1983 Plenum Press, New York
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Lakowicz, J.R. (1983). Phase-Sensitive Detection of Fluorescence. In: Principles of Fluorescence Spectroscopy. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7658-7_4
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DOI: https://doi.org/10.1007/978-1-4615-7658-7_4
Publisher Name: Springer, Boston, MA
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