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Channel Protein Engineering

An Approach to the Identification of Molecular Determinants of Function in Voltage-Gated and Ligand-Regulated Channel Proteins

  • Chapter
Ion Channels

Abstract

Transfer of information in the brain is mediated by the activity of ion-channel proteins. For several decades, these transducers were characterized by electrophysiological and biophysical techniques (for review see Hille, 1984).

Scientific knowledge comprises the world of the known and knowable, and hypotheses based upon the known are scientific hypotheses; the validity of science and its hypotheses rests upon proof by correspondence with reality. Beyond these there is only the great vacuity of the unknown....

Homer Smith, Kamongo, Viking Press, New York, 1956

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Abbreviations

AChR:

acetylcholine receptor

ACh:

acetylcholine

V:

applied voltage

γ:

single-channel conductance

VSSC:

voltage-sensitive sodium channel

GABA R:

γ-aminobutyric acid receptor

SCP:

synthetic channel peptide

Ï„ o :

open channel lifetime

Ï„ c :

closed channel lifetime

PCP:

phencyclidine

CPZ:

chlorpromazine

TTX:

tetrodotoxin

BTX:

batrachotoxin

µH :

hydrophobic moment.

A:

alanine

R:

arginine

N:

asparagine

D:

aspartate

C:

cysteine

E:

glutamate

Q:

glutamine

G:

glycine

H:

histidine

I:

isoleucine

L:

leucine

K:

lysine

M:

methionine

F:

phenylalanine

P:

proline

S:

serine

T:

threonine

W:

tryptophan

Y:

tyrosine

V:

valine.

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  1. Departments of Biology and Physics, University of California at San Diego, La Jolla, California, 92093-0319, USA

    Mauricio Montal

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  1. Northwestern University Medical School, Chicago, Illinois, USA

    Toshio Narahashi

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© 1990 Plenum Press, New York

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Montal, M. (1990). Channel Protein Engineering. In: Narahashi, T. (eds) Ion Channels. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7305-0_1

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