Summary
The TSRLM is a practical tool for practical microscopy problems: it gives a real image in real time and real color, and the recording process is direct photomicrography. This chapter represents the advantages of the TSRLM in allowing stereo images to be acquired directly. Through-focussing during photography, repeated twice on inclined axes, provides the simplest direct means of obtaining stereoscopic views at the limit of resolution in light microscopy.
The method will be limited mainly by the characteristics of real objects and objective lenses. The translucency of the object will be impaired in proportion to the density of the light-scattering features which it is hoped to visualise. High resolving power objectives have a short free-working distance and collide with the specimen or its cover glass when one has focussed down through that distance.
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References
Boyde, A. (1985a) The tandem scanning reflected light microscope. Part 2—Pre-Micro 84 applications at UCL. Proc Roy Microsc Soc 20, 131–139.
Boyde, A. (1985b) Stereoscopic images in confocal (tandem scanning) microscopy. Science 230, 1270–1272.
Boyde, A. (1987) Color-coded stereo images from the tandem scanning reflected light microscope (TSRLM). J. Microsc. 146, 137–142.
Boyde, A., Howell P.G.T., Franc F. (1986) A Simple SEM Stereophotogrammetric method for three dimensional evaluation of features on flat substrates, J. Micros 143: 257–264.
Brakenhoff, G.J., Van Der Voort, H.T.M., Van Spronsen, E.A., Linnemans, W.A.M., & Nanninga, N. (1985) Three-dimensional chromatin distribution in neuroblastoma nuclei shown by confocal scanning laser microscopy. Nature 317, 748–749.
Carlsson, K., Danielsson, P.E., Lenz, R., Liljeborg, A., Majloef, L. & Aslund, N. (1985) Three-dimensional microscopy using a confocal laser scanning microscope. Optics Letters 10, 53–55.
Cox, I.J. & Sheppard, C.J.R. (1983) Digital image processing of confocal images. Image and Vision Computing 1, 53.
Howard, V., Reid S.A., Baddeley, A. & Boyde A. (1985) Unbiased estimation of particle density in the tandem scanning reflected light microscope. J. Microsc. 138, 203–212.
Minsky, M. Microscopy Apparatus. United States Patent Office. Filed Nov. 7, 1957, granted Dec. 19, 1961. Patent No. 3,013,467.
Petran, M. and Hadravsky, M. (1968) Zpusob a zarizeni pro omezeni rozptylu svetla v mikroskopu pro osvetleni shora. Czechoslovak Patent No. 128936, application 5–7–66, granted 15–2–68, published 15–9–68.
Petran M., Hadravsky, M. & Boyde, A. (1985) The tandem scanning reflected light microscope. Scanning, 7, 97–108.
Sugimoto, S.A., & Ichioka, Y. (1985) Digital composition of images with increased depth of focus considering depth information. Applied Optics 24, 2076–2080.
Van der Voort, H.T.M., Brakenhoff, G.J., Valkenburg, J.A.C. & Nanninga, N. (1985) Design and use of a computer controlled confocal microscope for biological applications. Scanning 7, 66–78.
Wijnaendts Van Resandt, R.W., Marsman, H.J.B., Kaplan, R., Davoust, J., Stelzer, E.H.K. and Stricker, R. (1985) Optical fluorescence microscopy in three dimensions: microtomoscopy. J. Microsc. 138, 29–34.
Wilson, T. & Sheppard, C. (1984) Theory and practice of scanning optical microscopy. Academic Press—London 1984.
Wolf, R. (1989) A novel beam-splitting microscope tube for taking stereopairs with full resolution Nomarski optics: phase contrast; or epifluorescence. J Microsc. 153, 181–186.
Xiao, G.Q. & Kino, G.S. (1987) A real-time confocal scanning optical microscope, Proc. SPIE, Vol. 809, Scanning Imaging Technology, T. Wilson & L. Balk, Eds. 107–113 (1987)
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© 1990 Plenum Press, New York
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Boyde, A. (1990). Direct Recording of Stereoscopic Pairs Obtained From Disk-scanning Confocal Light Microscopes. In: Pawley, J.B. (eds) Handbook of Biological Confocal Microscopy. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7133-9_15
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DOI: https://doi.org/10.1007/978-1-4615-7133-9_15
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