Molecular Genetics of Yeast
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In general, bacterial and eukaryotic chromosomes are too large to be isolated easily, and cannot be analysed by the techniques which have been used so successfully with plasmids and virus chromosomes. One exception to this is mitochondrial DNA from yeast, molecular weight 50 million, which can be isolated and analysed in essentially a very similar fashion. Consequently the mitochondrial DNA of yeast is probably the most completely mapped and characterized piece of eukaryotic DNA. More recently, a second extrachromosomal molecule has been detected in yeast, the so-called 2µm circle, molecular weight 3.9 million. This has been used to transmit yeast genes into E. coli and back into yeast by a high-frequency transformation system. This chapter will discuss the mapping of the mitochondrial genome and the discovery of intervening sequences in genes. Finally, an account of transformation in yeast will be given.
KeywordsMitochondrial Genome Mating Type Hybrid Plasmid Opposite Mating Type Yeast Chromosome
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