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Molecular Genetics of Yeast

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Part of the Tertiary Level Biology book series (TLB)

Abstract

In general, bacterial and eukaryotic chromosomes are too large to be isolated easily, and cannot be analysed by the techniques which have been used so successfully with plasmids and virus chromosomes. One exception to this is mitochondrial DNA from yeast, molecular weight 50 million, which can be isolated and analysed in essentially a very similar fashion. Consequently the mitochondrial DNA of yeast is probably the most completely mapped and characterized piece of eukaryotic DNA. More recently, a second extrachromosomal molecule has been detected in yeast, the so-called 2µm circle, molecular weight 3.9 million. This has been used to transmit yeast genes into E. coli and back into yeast by a high-frequency transformation system. This chapter will discuss the mapping of the mitochondrial genome and the discovery of intervening sequences in genes. Finally, an account of transformation in yeast will be given.

Keywords

Mitochondrial Genome Mating Type Hybrid Plasmid Opposite Mating Type Yeast Chromosome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Blackie & Son Ltd 1987

Authors and Affiliations

  1. 1.King’s College LondonUK

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