Recombinant DNA Technology

Part of the Tertiary Level Biology book series (TLB)


The basic techniques of genetics have relied heavily on spontaneous or induced mutation, followed by standard recombination methods using sexual or asexual processes. There are natural barriers to limit recombination between different organisms, although some of these can be circumvented. Much interest has recently centred around techniques which avoid natural barriers to recombination. A wide variety of techniques has been used, including production of hybrids by cell or protoplast fusion, transfer of genes between different genera of bacteria by promiscuous plasmids, and extracellular manipulation of DNA from widely different sources.


Bacteriophage Lambda Ampicillin Resistance Hybrid Plasmid Replacement Vector Allele Replacement 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Arber, W. (1974) DNA modification and restriction. Prog. Nucleic Acid Res. and Molecular Biol. 14, 1–37.CrossRefGoogle Scholar
  2. Ashby Report (1975). Report of the working party on the experimental manipulation of the genetic composition of microorganisms. Cmnd. 5880, HMSO, London.Google Scholar
  3. Atherton, K. T., Byrom, D. and Dart, E. C. (1979) Genetic manipulations for industrial processes, in 29th Symposium of the Society for General Microbiology: Microbial Technology: Current State and Future Project (eds. A. T. Bull, D. C. Ellwood and C. Ratledge), Cambridge University Press, Cambridge, 379–405.Google Scholar
  4. Ball, C. (1984) Genetics and Breeding of Industrial Microorganisms, CRC Press, Boca Raton.Google Scholar
  5. Clewell, D. B. and Helsinki, D. R. (1969) Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an open circular form. Proc. Nat. Acad. Sci. Wash. 62, 1159–1166.CrossRefGoogle Scholar
  6. Cohen, S. N. (1975) The manipulation of genes. Sci. Amer. 233(1), 24–33 (reprinted in Recombinant DNA, 1978, ed. D. Freifelder).CrossRefGoogle Scholar
  7. Cohen, S. N., Chang, A. C. Y., Boyer, H. W. and Helling, R. B. (1973) Construction of biologically functional bacterial plasmids in vitro. Proc. Nat. Acad. Sci. Wash. 70, 3240–3244.CrossRefGoogle Scholar
  8. Glover, D. M. (1984) Gene Cloning: The Mechanics of DNA Manipulation, 2nd edn., Chapman and Hall, London.Google Scholar
  9. Guerry, P., Le Banc, D. J. and Falkow, S. (1973) General method for the isolation of plasmid deoxyribonucleic acid. J. Bact. 116, 1064–1066.Google Scholar
  10. Kornberg, A. (1980) DNA replication plus supplement (1982) W. H. Freeman and Co., San Francisco.Google Scholar
  11. Lewin, B. (1977) Gene Expression, vol. 3, Plasmids and Phages, Wiley-Interscience, New York.Google Scholar
  12. Luria, S. E. (1953) Host-induced modifications of bacterial viruses. Cold Spring Harb. Symp. Quant. Biol. 18, 237.CrossRefGoogle Scholar
  13. Maniatis, T., Fritsch, E. J. and Sambrook, J. (1982) Molecular Cloning: a Laboratory Manual Cold Spring Harbor Laboratory, Cold Spring Harbor.Google Scholar
  14. Murray, N. E. (1983) Phage lambda and molecular cloning, in Lambda II (eds. R. W. Hendrix, J. W. Roberts, F. W. Stahl and R. A. Weisberg), Cold Spring Harbor Laboratory, Cold Spring Harbor.Google Scholar
  15. Old, R. W. and Primrose, S. B. (1985) Principles of Gene Manipulation, 3rd edn., Blackwell Scientific, Oxford.Google Scholar
  16. Peberdy, J. F., Eyssen, H. and Anne, J. (1977) Interspecific hybridisation between Penicillium chrysogenum and Penicillium cyaneo-fulvum following protoplast fusion. Mol. gen. Genet. 157, 281–284.CrossRefGoogle Scholar
  17. Postgate, J. R. and Cannon, F. C. (1981) The molecular and genetic manipulation of nitrogen fixation. Phil. Trans. Roy. Soc. London Ser. B 292, 589–599.CrossRefGoogle Scholar
  18. Roberts, R. J. (1978) in Microbiology 1978 (ed. D. Schlesinger), American Society for Microbiology, Washington, 5–9.Google Scholar
  19. Shortle, D., DiMaio, D. and Nathans, D. (1981) Directed mutagenesis. Ann. Rev. Genetics 15, 265–294.CrossRefGoogle Scholar
  20. Stent, G. (1971) Molecular Genetics, 1st edn., W. H. Freeman, San Francisco.Google Scholar
  21. Vandegrift, V. (1979) Recombinant DNA: scientific and social perspectives. J. chem. Educ. 56, 77–82.CrossRefGoogle Scholar
  22. Williams Report (1976). Report of the working party on the practice of gene manipulation. Cmnd. 6600, HMSO, London.Google Scholar
  23. Windass, J. D., Worsey, M. J., Pioli, E. M., Barth, P. T., Atherton, K. T., Dart, E. C., Byrom, D., Powell, K. and Senior, P. J. (1980) Improved conversion of methanol to single cell protein by Methylophilus methylotrophus. Nature, London 287, 396–401.CrossRefGoogle Scholar

Copyright information

© Blackie & Son Ltd 1987

Authors and Affiliations

  1. 1.King’s College LondonUK

Personalised recommendations