The Analysis of Blood Lipids by Chromatography and Infrared Spectrophotometry
The great interest in the “blood lipids in recent years particularly in relationship to a number of disease states has stimulated the development of new methods for the analysis of these compounds. Many of the older procedures did not resolve many of the blood lipid components but measured only groups or classes in toto. This is particularly true for phospholipids in whole blood, plasma, and erythrocytes. It is obvious now that there are specific changes occurring within the specific lipid classes which may well go undetected by older methods of analysis (1–3). This presentation will describe methods which have been developed for analyzing most if not all of the individual species of lipids present in blood, both in the formed elements and plasma. These procedures involve Sephadex column chromatography and thin-layer chromatography coupled with infrared spectrophotometry and gas-liquid chromatography.
KeywordsPhosphatidic Acid Total Lipid Extract Phosphatidyl Inositol Sheep Erythrocyte Glyceryl Ether
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