Advertisement

Environmental Effects on the Structure of Human Serum High Density Lipoprotein and Its Apoprotein

  • Angelo M. Scanu
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 4)

Abstract

Previous studies from this laboratory using the technique of optical rotatory dispersion (ORD) * have indicated that the human serum density lipoprotein ciass floating between solvent densities of 1.063–1.21 has a high content in a-helïx, which is to a large extent retained after the lipoprotein is exhaustively extracted with a mixture of ethanol-ether (1,2). A relatively small influence on the optical rotatory dispersion parameters was noted after relipidation of the lipid-free apoprotein (apo HDL) with aqueous dispersions of phospholipids (3,4). Although the results gave sufficient evidence that the overall secondary structure of apo HDL is relatively independent of bound lipids, the possibility remained that conformational differences between lipid-rich and lipid-poor forms of apo HDL did exist, but could not be detected under the experimental conditions employed. The availability of the technique of circular dichroism (CD), which has the advantage over ORD of more clearly resolving bands relative to the optically active chromophores in the ultraviolet and far ultraviolet regions (7) offered the opportunity of reexamining this question. Under the chosen conditions, the CD spectrum of HDL was found to differ significantly from that of its lipid-poor product. Distinction between these two forms was also observed in terms of their sensitivity to chemical modification of the protein and changes of the nature and temperature of the solvent medium.

Keywords

Circular Dichroism Circular Dichroism Spectrum Aqueous Dispersion Succinic Anhydride Optical Rotatory Dispersion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

HDL3

human serum high density lipoprotein of d 1.125–1.21

s-HDL3

succinylated HDL3

apo HDL3

apo-protein of HDL3 obtained by organic solvent extraction

s-spo HDL3

succinylated apo HDL3

SDS

sodium dodecylsulphate

G-HCl

guanidine hydrochloride

ORD

optical rotatory dispersion

CD

circular dichroism

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Seanu, A. Proc. Natl. Acad. Sci.,U.S. 54: 1699(1965).CrossRefGoogle Scholar
  2. 2.
    Scanu, A. and Granda, J. L. Prog. Biochem. Pharmacol 4: 153 (1968).Google Scholar
  3. 3.
    Scanu, A. J. Biol. Chem. 242: 711 (1967).PubMedGoogle Scholar
  4. 4.
    Scanu, A. “Proceedings of the American Meat Industry Research Conference”, American Meat Institute Foundation, Chicago, 1966, pp. 19–26.Google Scholar
  5. 5.
    Scanu, A. and Hirz, R. J. Amer. Oil Chem. Soc. 45: 110A (1968).Google Scholar
  6. 6.
    Scanu, A. and Hirz, R. Proc. Natl. Acad. Sci., U.S. 59: 890 (1968).CrossRefGoogle Scholar
  7. 7.
    Beychok, S. Science 154: 1288(1966).PubMedCrossRefGoogle Scholar
  8. 8.
    Scanu, A. and Granda, J. L. Biochemistry 5: 446(1966).PubMedCrossRefGoogle Scholar
  9. 9.
    Avîgan, J. J. Biol. Chem. 226: 957(1957)7PubMedGoogle Scholar
  10. 10.
    Scanu, A. J. Lipid Res. 7: 295 (1966).PubMedGoogle Scholar
  11. 11.
    Scanu, A., Reader, W. and Edelstein, C. Biochim. Biophys. Acta 160: 32 (1968).PubMedGoogle Scholar
  12. 12.
    Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. J. Biol. Chem. 193: 265 (1951).PubMedGoogle Scholar
  13. 13.
    Abell, L. L, Levy, B. B., Brodie, B. B. and Kendall, F. E. J. Biol. Chem. 195: 357(1952).Google Scholar
  14. 14.
    Fiske, C. H. and SubbaRow, Y. J. Biol. Chem. 66: 375 (1925).Google Scholar
  15. 15.
    Van Handel, E. and Zilversmit, D. B. J. Lab. Clin. Med. 50: 152 (1957).Google Scholar
  16. 16.
    Sodhi, H. S. and Gould, R. G. J. Biol. Chem. 244: 1205 (1967).Google Scholar
  17. 17.
    Holzwarth, G. and Doty, P. J. Amer. Chem. Soc. 87: 218 (1965).CrossRefGoogle Scholar
  18. 18.
    Scanu, A. Advan. Lipid Res. 7: 63 (1966).Google Scholar
  19. 19.
    Sanbar, S. S. and Alaupovic, P. Biochim. Biophys. Acta 71: 235 (1963).PubMedCrossRefGoogle Scholar
  20. 20.
    Shore, V. and Shore, B. Biochemistry 6: 1962 (1967).PubMedCrossRefGoogle Scholar
  21. 21.
    Jirgensons, B. J. Biol. Chem. 241: 147(1966).PubMedGoogle Scholar
  22. 22.
    Scanu, A., In preparationGoogle Scholar

Copyright information

© Pleum Press 1969

Authors and Affiliations

  • Angelo M. Scanu
    • 1
  1. 1.Departments of Medicine and Biochemistry and Argonne Cancer Research HospitalUniversity of ChicagoChicagoUSA

Personalised recommendations