Some Factors Affecting Release of Clearing Factor Lipase from Isolated Perfused Rat Heart
The presence of a lipolytic enzyme has been demonstrated in a variety of tissue preparations of the heart (2–10). This enzyme has been referred to rather indiscriminately as “lipoprotein lipase,” a term which was introduced by Korn (2). According to Korn this lipase was extracted from rat heart acetone powder in an ammonia buffer in the presence of heparin; the enzyme had little activity unless the substrate which was a coconut oil emulsion (Ediol) was “activated” by serum.
KeywordsLipase Activity Lipolytic Activity Perfusion Fluid Alloxan Diabetes Buffer Fluid
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