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Use of Electron Microscopy in Virology

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Comprehensive Virology

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Abstract

It is now 40 years since the first electron micrographs of tobacco mosaic virus were published (Kausche et al., 1939). The affection which virologists have had for the electron microscope and electron microscopists for virus specimens since that time has been extremely fruitful. As evidence of the anticipated productivity of electron microscopy, consider the following quotation from p. 274 of the second edition of the book by Burton and Kohl (1946):

Stanley and Anderson state as their considered opinion: “Although excellent micrographs of bacteria have been obtained by means of this apparatus and have proved of value in supplementing information already available, it would appear that the electron microscope will be of greatest value in the microscopy of objects having sizes between 5 and 250 mμ, a range not covered by the light microscope and one in which practically all viruses have been found to fall. The electron microscope offers the possibility of securing micrographs of individual virus particles and thus of establishing their sizes and shapes with some precision. It should also be possible to determine the extent of the variation in the size and shape of a given virus and even perhaps learn something of the mechanism by means of which a virus particle is duplicated within the host and of the nature of the difference between strains of a given virus.”

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  1. Department of Biochemistry and Biophysics, University of Rhode Island, Kingston, Rhode Island, 02881, USA

    Harold W. Fisher

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  1. Department of Molecular Biology and Virus Laboratory, University of California, Berkeley, California, USA

    Heinz Fraenkel-Conrat

  2. Department of Microbiology, University of Virginia, Charlottesville, Virginia, USA

    Robert R. Wagner

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Fisher, H.W. (1981). Use of Electron Microscopy in Virology. In: Fraenkel-Conrat, H., Wagner, R.R. (eds) Comprehensive Virology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-6693-9_2

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