Abstract
The technique of RT-PCR has become a powerful method for studying gene expression in various species and tissues. Here we present a competitive PCR for quantitative measurement of absolute levels of mRNA molecules for the regulatory α2/δ subunit of the high-voltage-activated dihydropyridine sensitive human cardiac L-type calcium channel. With the method described the dynamic process of cardiac gene expression can be studied in human endomyocardial biopsies in different patient collectives and even in the individual. (Mol Cell Biochem 172: 89–95, 1997)
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© 1997 Springer Science+Business Media Dordrecht
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Hullin, R., Asmus, F., Steinbeck, G. (1997). Competitive RT-PCR for studying gene expression in micro biopsies. In: Pierce, G.N., Claycomb, W.C. (eds) Novel Methods in Molecular and Cellular Biochemistry of Muscle. Developments in Molecular and Cellular Biochemistry, vol 20. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-6353-2_9
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DOI: https://doi.org/10.1007/978-1-4615-6353-2_9
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