Abstract
In order to test the hypothesis that mitochondrial transcription factor A (mtTFA) regulates mitochondrial transcription in vivo, mtTFA was overexpressed in HeLa cells and imported into isolated rat liver mitochondria. Five hours after transfection with an eukaryotic expression vector, mitochondrial transcripts for cytochrome-c-oxidase subunit I and 12 S rRNA were increased over controls. In the presence of rat liver mitochondria, the 29 kDa mtTFA, generated by in vitro translation, was processed to a 24 kDa protein which was protected from protease digestion. This demonstrates that mtTFA was imported into the matrix. Incorporation of 32P-UTP into mitochondrial transcripts was stimulated following import of mtTFA. We conclude that the intracellular and intramitochondrial concentration of mtTFA, respectively, indeed regulates mitochondrial transcription. (Mol Cell Biochem 174: 227–230, 1997)
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References
Shadel G, Clayton DA: Mitochondrial transcription initiation. J Biol Chem 268: 16083–16086, 1993
Parisi MA, Xu B, Clayton DA: A human mitochondrial transcriptional activator can functionally replace a yeast mitochondrial HMG-box protein both in vivo and in vitro. Mol Cell Biol 13: 1951–1961, 1993
Wiesner RJ, Kurowski TT, Zak R: Regulation by thyroid hormone of nuclear and mitochondrial genes encoding subunits of cytochrome-c oxidase in rat liver and skeletal muscle. Mol Endo 6: 1458–1467, 1992
Wiesner RJ, Aschenbrenner V, Ruegg JC, Zak R: Coordination of nuclear and mitochondrial gene expression during the development of cardiac hypertrophy in rats. Am J Physiol 267: C229–C235, 1994
Klingenspor M, Ivemeyer M, Wiesinger H, Haas K, Heldmaier G, Wiesner RJ: Biogenesis of thermogenic mitochondria in brown adipose tissue of Djungarian during cold adaptation. Biochem J 316: 607–613, 1996
Garstka HL, Facke M, Escribano JR, Wiesner RJ: Stoichiometry of mitochondrial transcripts and regulation of gene expression by mitochondrial transcription factor A. Biochem Biophys Res Comm 200: 619–626, 1994
Parisi MA, Clayton DA: Similarity of human mitochondrial transcription factor 1 to high mobility group proteins. Science 252: 965–969, 1991
Rittner K, Stöppler H, Pawlita M, Sczakiel G: Versatile eukaryotic vectors for strong and constitutive transient and stable gene expression. Methods Mol Cell Biol 2: 176–181, 1991
Enriquez JA, Lopez-Pérez MJ, Montoya J: Saturation of the processing of newly synthesized rRNA in isolated brain mitochondria. FEBS Lett 280: 32–36, 1991
Attardi G, Montoya J: Analysis of human mitochondrial RNA. In: S Fleischer, B Fleischer (eds). Biomembranes. Meth Enzymol 97: pp 435–469, 1983
Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning — A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, 1989
Walters MC, Fiering S, Eidemiller J, Magis W, Groudine M, Martin DIK: Enhancers increase the probability but not the level of gene expression. Proc Natl Acad Sci USA 92: 7125–7129, 1995
Nagley P: Coordination of gene expression in the formation of mammalian mitochondria. Trends Genet 7: 1–4, 1991
Wiesner RJ: Regulation of mitochondrial gene expression: transcription versus replication. Trends Genet (8): 264–265, 1992
Nagley P: Reply. Trends Genet 8(8) 265, 1992
Ghivizzani SC, Madsen CS, Nelen MR, Ammini CV, Hauswirth WW: In organello footprint analysis of human mitochondrial DNA: Human mitochondrial transcription factor A interactions at the origin of replication. Mol Cell Biol 14: 7717–7730, 1994
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© 1997 Springer Science+Business Media Dordrecht
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Montoya, J., Perez-Martos, A., Garstka, H.L., Wiesner, R.J. (1997). Regulation of mitochondrial transcription by mitochondrial transcription factor A. In: Gellerich, F.N., Zierz, S. (eds) Detection of Mitochondrial Diseases. Developments in Molecular and Cellular Biochemistry, vol 21. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-6111-8_35
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DOI: https://doi.org/10.1007/978-1-4615-6111-8_35
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