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Inhibition of Porcine Renal Ecto-ATP Diphosphohydrolase by Ca2+ Channel Blockers and Neuroleptic Drugs

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Ecto-ATPases

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Abstract

Brush-border membrane vesicles (BBMV) from rat, rabbit and pig kidneys have been used to characterize renal ecto-ATPases. In rat1,2 and rabbit renal BBMV3, Mg2+ stimulated enzyme activity better than Ca2+, whereas in vesicles from pig kidney the reverse order was found.4,5 The ecto-ATPases from all species hydrolysed purine and pyrimidine nucleotides with ITP being the most effectively cleaved substrate in rat and rabbit renal BBMV,1, 2, 6 and ATP in porcine renal BBMV.5 In most studies, the Km for ATP was found in the range between 0.14 and and 0.4 mM.2–4, 6, 7 Vanadate, ouabain, azide, oligomycin, N-ethylmaleimide, and bafilomycin hardly decreased the activity of renal ecto-ATPases in concentrations sufficient to block ion-transporting ATPases.1, 3, 5 Here we report on the inhibition by Ca2+ channel blockers and phenothiazines as well as on the differential effect of Ca2+ and Mg2+ on the ecto-ATP diphosphohydrolase in pig kidney BBMV.

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Authors and Affiliations

  1. Zentrum Physiologie und Pathophysiologie, Humboldtallee 23, D-37073, Göttingen, Germany

    Jens Rengelshausen, Christa Schweickhardt & Gerhard Burckhardt

Authors
  1. Jens Rengelshausen
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  2. Christa Schweickhardt
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  3. Gerhard Burckhardt
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Editor information

Editors and Affiliations

  1. University of Aarhus, Aarhus C, Denmark

    Liselotte Plesner

  2. University of Cincinnati, Cincinnati, Ohio, USA

    Terence L. Kirley

  3. University of California, Los Angeles, California, USA

    Aileen F. Knowles

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© 1997 Springer Science+Business Media New York

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Rengelshausen, J., Schweickhardt, C., Burckhardt, G. (1997). Inhibition of Porcine Renal Ecto-ATP Diphosphohydrolase by Ca2+ Channel Blockers and Neuroleptic Drugs. In: Plesner, L., Kirley, T.L., Knowles, A.F. (eds) Ecto-ATPases. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5955-9_13

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  • DOI: https://doi.org/10.1007/978-1-4615-5955-9_13

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-7729-0

  • Online ISBN: 978-1-4615-5955-9

  • eBook Packages: Springer Book Archive

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