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Neurobiology pp 109–128Cite as

Comparison of “Near Membrane” and Bulk Cytoplasmic Calcium Concentration in Single Cardiac Ventricular Myocytes During Spontaneous Calcium Waves

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Part of the book series: NATO ASI Series ((NSSA,volume 289))

Abstract

In this paper we discuss differences in the time course of changes of intracellular calcium concentration ([Ca2+]i) occurring in the bulk cytoplasm and adjacent to the surface membrane (subsarcolemmal or ‘fuzzy’ space) during spontaneous oscillatory release of Ca2+ from the sarcoplasmic reticulum (SR). Sarcolemmal Na-Ca exchange current and [Ca2+]i were measured in single voltage clamped rat ventricular myocytes.

Spontaneous Ca2+ release from the SR resulted in a transient inward current which developed and decayed more quickly than the corresponding changes in [Ca2+]i measured using the Ca2+ sensitive fluorescent indicators Indo-1, Fluo-3 and Calcium Green-1. The discrepancy in the time course of changes in current and [Ca2+]i results in a hysteresis between [Ca2+]i and current.

A similar hysteresis was observed if [Ca2+]i was raised with caffeine. The hysteresis between current and [Ca2+]i was removed by low pass filtering the current record with a time constant of 132 ms.

Digital video imaging was performed to allow simultaneous measurement of [Ca2+]i at all points of the cell during spontaneous release of Ca2+ from the SR. The hysteresis between current and [Ca2+]i remained even after the spatial and temporal properties of the Ca2+ wave and any non linear relationship between current and fluorescence and [Ca2+]i were accounted for.

Using a model in which there is a barrier to diffusion of Ca2+ between the subsarcolemmal and bulk compartments the hysteresis between current and [Ca2+]i can be accounted for. The calculated subsarcolemmal Ca2+ concentration rises before, and to a higher level than the measured bulk cytoplasmic Ca2+ concentration. The delay introduced by this diffusion barrier is equivalent to a time constant of 133 ms.

The subsarcolemmal space introduced in this paper may be equivalent to the ‘fuzzy space’ previously suggested to be important in controlling SR Ca2+ release.

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Author information

Authors and Affiliations

  1. Department of Veterinary Preclinical Science, The University of Liverpool, P.O. Box 147, Liverpool, L69 3BX, UK

    A. W. Trafford, M. E. Díaz, S. C. O’Neill & D. A. Eisner

Authors
  1. A. W. Trafford
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  2. M. E. Díaz
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  3. S. C. O’Neill
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  4. D. A. Eisner
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Editor information

Editors and Affiliations

  1. Università di Genova, Genova, Italy

    Vincent Torre

  2. Consiglio Nazionale delle Richerche (CNR), Genova, Italy

    Franco Conti

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© 1996 Springer Science+Business Media New York

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Trafford, A.W., Díaz, M.E., O’Neill, S.C., Eisner, D.A. (1996). Comparison of “Near Membrane” and Bulk Cytoplasmic Calcium Concentration in Single Cardiac Ventricular Myocytes During Spontaneous Calcium Waves. In: Torre, V., Conti, F. (eds) Neurobiology. NATO ASI Series, vol 289. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5899-6_9

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  • DOI: https://doi.org/10.1007/978-1-4615-5899-6_9

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-7706-1

  • Online ISBN: 978-1-4615-5899-6

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