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Structural and Functional Studies on the Heart Fatty Acid-Binding Protein

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Frontiers in Bioactive Lipids

Abstract

In the past we surveyed many aspects of cytoplasmic fatty acid-binding proteins (FABPs)1-4. These molecules with relatively low molecular masses (14-15 kDa) are supposed to play a role in fatty acid transfer and targeting, and in fatty acid metabolism. A second function is their possible modulatory role on the effect of fatty acids on gene expression, receptors, metabolic enzymes and signal transduction. An indirect involvement of FABP in modulation of cell growth and differentiation was suggested for hepatocytes, cardiomyocytes and mammary epithelial cells.

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References

  1. J.H. Veerkamp, R.A. Peeters, and R.G.H.J. Maatman, Structural and functional features of cytoplasmic fatty acid-binding proteins, Biochim. Biophys. Acta 1081:1 (1991).

    Article  PubMed  CAS  Google Scholar 

  2. J.H. Veerkamp, T.H.M.S.M. van Kuppeveit, R.G.H.J. Maatman, and C.F.M. Prinsen, Structural and functional aspects of cytosolic fatty acid-binding proteins, Prostaglandins Leukot. Essent. Fatty Acids 49:887 (1993).

    Article  PubMed  CAS  Google Scholar 

  3. J.H. Veerkamp and R.G.H.J. Maatman, Cytoplasmic fatty acid-binding proteins: their structure and genes, Prog. Lipid Res. 34:17 (1995)

    Article  PubMed  CAS  Google Scholar 

  4. J.H. Veerkamp, Fatty acid transport and fatty acid-binding protein, Proc. Nutrition Soc. 54:23 (1995)

    Article  CAS  Google Scholar 

  5. R.G.H.J. Maatman, H.T.B. van Moerkerk, I.M.A. Nooren, E.J.J. van Zoelen, and J.H. Veerkamp, Expression of human liver fatty acid-binding protein in Escherichia coli and comparative analysis of its binding characteristics with muscle fatty acid-binding protein, Biochim. Biophys. Acta 1214:1 (1994).

    Article  PubMed  CAS  Google Scholar 

  6. B. Rolf, E. Oudenampsen-Krüger, T. Börchers, N.J. Faergeman, J. Knudsen, A. Lezius, and F. Spener, Analysis of the ligand binding properties of recombinant bovine liver-type fatty acid binding protein, Biochim. Biophys. Acta 1259:245 (1995).

    Article  PubMed  Google Scholar 

  7. G.V. Richieri, R.T. Ogata, and A.M. Kleinfeld, Equilibrium constants for the binding of fatty acids with fatty acid-binding proteins from adipocyte, intestine, heart, and liver measured with the fluorescent probe ADIFAB, J. Biol. Chem. 269:23918 (1994).

    PubMed  CAS  Google Scholar 

  8. R.G.H.J. Maatman, M. Degano, H.T.B. van Moerkerk, W.J.A. van Marrewijk, D.J. van der Horst, J.C. Sacchettini, and J.H. Veerkamp, Primary structure and binding characteristics of locust and human muscle fatty-acid-binding proteins, Eur. J. Biochem. 221:801 (1994)

    Article  PubMed  CAS  Google Scholar 

  9. J.M. LaLonde, M.A. Levenson, J.J. Roe, D.A. Bernlohr, and L.J. Banaszak, Adipocyte lipid-binding protein complexed with arachidonic acid, J. Biol. Chem. 269:25339 (1994).

    Google Scholar 

  10. K.R. Miller and D.P. Cistola, Titration calorimetry as a binding assay for lipid-binding proteins, Mol. Cell. Biochem. 123:29 (1993)

    Article  PubMed  CAS  Google Scholar 

  11. A.C.M. Young, G. Scapin, A. Kromminga, S.B. Patel, J.H. Veerkamp, and J.C. Sacchettini, Structural studies on human muscle fatty acid binding protein at 1.4Ã… resolution: binding interactions with three C18 fatty acids, Structure 2:523 (1994).

    Article  PubMed  CAS  Google Scholar 

  12. G. Zanotti, G. Scapin, P. Spadon, J.H. Veerkamp, and J.C. Sacchettini, Three-dimensional structure of recombinant human muscle fatty acid-binding protein, J. Biol. Chem. 267:18541 (1992).

    PubMed  CAS  Google Scholar 

  13. L. Banaszak, N. Winter, Z. Xu, D.A. Bernlohr, S. Cowan, and T.A. Jones, Lipid-binding proteins: a family of fatty acid and retinoid transport proteins, Adv. Protein Chem. 45:89 (1994).

    Article  PubMed  CAS  Google Scholar 

  14. G.V. Richieri, R.T. Ogata, and A.M. Kleinfeld, Thermodynamics of fatty acid binding to fatty acid-binding proteins and fatty acid partition between water and membranes measured using the fluorescent probe ADIFAB, J. Biol. Chem. 270:15076 (1995).

    Article  PubMed  CAS  Google Scholar 

  15. C.F.M. Prinsen and J.H. Veerkamp, Fatty acid binding and conformational stability of mutants of human muscle fatty acid-binding protein, Biochem. J. 314:253 (1996)

    PubMed  CAS  Google Scholar 

  16. J. Eads, J.C. Sacchettini, A. Kromminga, and J.I. Gordon, Escherichia coli-derived rat intestinal fatty acid binding protein with bound myristate at 1.5 Å resolution and I-FABPArg106→Gln with bound oleate at 1.74 Å resolution, J. Biol. Chem. 268:26375 (1993).

    PubMed  CAS  Google Scholar 

  17. R.S. Sha, C.D. Kane, Z. Xu, L.J. Banaszak, and D.A. Bernlohr, Modulation of ligand binding affinity of the adipocyte lipid-binding protein by selective mutation, J. Biol. Chem. 268:7885 (1993).

    PubMed  CAS  Google Scholar 

  18. A.E.A. Thumser, C. Evans, A.F. Worrall, and D.C. Wilton, Effect on ligand binding of arginine mutations in recombinant rat liver fatty acid-binding protein, Biochem. J. 297:103 (1994)

    PubMed  CAS  Google Scholar 

  19. M.G. Jakoby, K.R. Miller, J.J. Toner, A. Bauman, L. Cheng, E. Li, and D.P. Cistola, Ligand-protein electrostatic interactions govern the specificity of retinol-and fatty acid-binding proteins, Biochemistry 32:872 (1993).

    Article  PubMed  CAS  Google Scholar 

  20. F.M. Herr, J. Aronson, and J. Storch, Role of portal region lysine residues in electrostatic interactions between heart fatty acid binding protein and phospholipid membranes, Biochemistry 35:1296 (1996).

    Article  PubMed  CAS  Google Scholar 

  21. A.E.A. Thumser and D.C. Wilton, Characterization of binding and structural properties of rat liver fatty-acid-binding protein using tryptophan mutants, Biochem. J. 300:827 (1994)

    PubMed  CAS  Google Scholar 

  22. A.E.A. Thumser, J. Voysey, and D.C. Wilton, Mutations of recombinant rat liver fatty acid-binding protein at residues 102 and 122 alter its structural integrity and affinity for physiological ligands, Biochem. J. 314:943 (1996)

    PubMed  CAS  Google Scholar 

  23. L.J. Baier, J.C. Sacchettini, W.C. Knowler, J. Eads, G. Paolisso, P.A. Tataranni, H. Mochizuki, P.H. Bennett, C. Bogardus, and M. Prochazka, An amino acid substitution in the human intestinal fatty acid binding protein is associated with increased fatty acid binding, increased fat oxidation, and insulin resistance, J. Clin. Invest. 95:1281 (1995)

    Article  PubMed  CAS  Google Scholar 

  24. I.J. Ropson and C. Frieden, Dynamic NMR spectral analysis and protein folding: identification of a highly populated folding intermediate of rat intestinal fatty acid-binding protein by 19F NMR, Proc. Natl. Acad. Sci USA 89:7222 (1992).

    Article  PubMed  CAS  Google Scholar 

  25. N. Jiang and C. Frieden, Intestinal fatty acid binding protein: characterization of mutant proteins containing inserted cysteine residues, Biochemistry 32:11015 (1993).

    Article  PubMed  CAS  Google Scholar 

  26. Y. Yang, E. Spitzer, N. Kenney, W. Zschiesche, M. Li, A. Kromminga, T. Müller, F. Spener, A. Lezius, J.H. Veerkamp, G.H. Smith, D.S. Salomon, and R. Grosse, Members of the fatty acid binding protein family are differentiation factors for the mammary gland, J. Cell Biol. 127:1097 (1994)

    Article  PubMed  CAS  Google Scholar 

  27. R.A. Peeters, J.H. Veerkamp, and R.A. Demel, Are fatty acid-binding proteins involved in fatty acid transfer?, Biochim. Biophys. Acta 1002:8 (1989).

    Article  PubMed  CAS  Google Scholar 

  28. H.T. Huynh, C. Larsson, S. Narod, and M. Pollak, Tumor suppressor activity of the gene encoding mammary-derived growth inhibitor, Cancer Res. 55:2225 (1995)

    PubMed  CAS  Google Scholar 

  29. B. Binas, B. Gusterson, R. Wallace, and A.J. Clark, Epithelial proliferation and differentiation in the mammary gland do not correlate with cFABP gene expression during early pregnancy, Dev. Genet. 17:167 (1995)

    Article  PubMed  CAS  Google Scholar 

  30. P.B.J. Burton, C.E. Hogben, C.L. Joannou, A.G.B. Clark, J.J. Hsuan, N.F. Totty, C. Sorensen, R.W. Evans, and M.J. Tynan, Heart fatty acid binding protein is a novel regulator of cardiac myocyte hypertrophy, Biochem. Biophys. Res. Commun. 205:1822 (1994)

    Article  PubMed  CAS  Google Scholar 

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Veerkamp, J.H., Prinsen, C.F.M. (1996). Structural and Functional Studies on the Heart Fatty Acid-Binding Protein. In: Vanderhoek, J.Y. (eds) Frontiers in Bioactive Lipids. GWUMC Department of Biochemistry and Molecular Biology Annual Spring Symposia. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5875-0_7

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  • DOI: https://doi.org/10.1007/978-1-4615-5875-0_7

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-7694-1

  • Online ISBN: 978-1-4615-5875-0

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