Skip to main content
SpringerLink
Log in

Lipopolysaccharide-, Liposome-Encapsulated MTP-PE- and Dexamethasone- Regulated Prostaglandin Release in Rat Liver Macrophages: Role of Cytosolic Phospholipase A2 and Prostaglandin H Synthase-2

  • Chapter
Book cover Frontiers in Bioactive Lipids

Abstract

Rat liver macrophages (RLM), activated by a variety of agents, are known to release various eicosanoids including prostaglandin (PG)E2 and PGD2 1. The formation of prostanoids is strictly dependent on Ca2+ and is thought to be determined by the activation of a phospholipase (PL)A2 2 and the expression of prostaglandin H synthase (PGHS)-2. It has been shown that RLM express an intracellular cPLA2 which is strictly Ca2+-dependent in the range from 10-7-10-6 M and which shows a Ca2+-dependent translocation to cellular membranes3,4. cPLA2 phosphorylation has been shown to be most likely catalysed by mitogen activated protein (MAP) kinase4. Further evidence has been provided that in addition to regulation by cPLA2, release of prostanoids in RLM is also dependent on the expression of PGHS-25.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. P. Dieter, A.Schulze-Specking, and K.Decker, Differential inhibition of prostaglandin and Superoxide production by dexamethasone in primary cultures of rat Kupffer cells, Eur. J. Biochem. 159: 451 (1986)

    Article  PubMed  CAS  Google Scholar 

  2. P. Dieter, A. Schulze-Specking, and K. Decker, Ca2+ requirement of prostanoid but not Superoxide production by rat Kupffer cells, Eur. J. Biochem. 177: 61 (1993)

    Google Scholar 

  3. H. Krause, P. Dieter, A. Schulze-Specking, A. Ballhorn, and K. Decker. Ca2+-induced reversible translocation of PLA2 between the cytosol and the membrane fraction of rat liver macrophages, Eur. J. Biochem. 199: 355 (1991).

    Article  PubMed  CAS  Google Scholar 

  4. P. Ambs, M. Baccarini, E. Fitzke, and P. Dieter, Role of cytosolic phospholipase A2 in arachidonic acid release of rat-liver macrophages: regulation by Ca2+ and phosphorylation, Biochem. J. 311: 189 (1995)

    PubMed  CAS  Google Scholar 

  5. F. Zhang, U. Warskulat, M. Wettstein, R. Schreiber, H. Henninger, K. Decker, and D. Häussinger, Hyperosmolarity stimulates prostaglandin synthesis and cyclooxygenase-2 expression in activated rat liver macrophages, Biochem. J. 312: 135 (1995)

    PubMed  CAS  Google Scholar 

  6. P. Dieter, P. Ambs, E. Fitzke, H. Hidaka, and H. Schwende, Comparative studies of cytotoxicity and the release of TNF-α, nitric oxide, and eicosanoids in liver macrophages treated with lipopolysaccharide and liposome encapsulated MTP-PE, J. Immunol. 155: 2595 (1995)

    PubMed  CAS  Google Scholar 

  7. J. Duyster, H. Schwende, E. Fitzke, H. Hidaka, and P. Dieter, Different roles of PKC-β and PKC-δ in AA-cascade, Superoxide formation and phosphoinositide hydrolysis, Biochem. J. 292: 203 (1993).

    PubMed  CAS  Google Scholar 

  8. J.D. Clark, L.L. Lin, R.W. Kriz, S.R. Chakkodabylu, L.A. Sultzman, A.Y. Lin, N. Milona, and J.L. Knopf, A novel arachidonic acid-selective cPLA2 contains a Ca2+-dependent translocation domain with homology to PKC and GAP, Cell 65: 1043 (1991)

    Article  PubMed  CAS  Google Scholar 

  9. A. Habib, C. Créminon, Y. Frobert, J.H. Grassi, P. Pradelles, and J. MacLouf, Demonstration of an inducible cyclooxygenase in human endothelial cells using antibodies raised against the carboxyl-terminal region of the cyclooxygenase-2, J. Biol Chem., 268: 23448 (1993)

    PubMed  CAS  Google Scholar 

  10. Th. Peters, U. Karck, and K. Decker, Interdependence of tumor necrosis factor, prostaglandin E2, and protein synthesis in lipopolysaccharide-exposed kupffer-cells, Eur. J. Biochem. 191: 583 (1990)

    Article  PubMed  CAS  Google Scholar 

  11. H.R. Herschman, Prostaglandin Synthase 2, Biochim. Biophys. Acta, 1299: 125 (1996)

    Article  PubMed  Google Scholar 

  12. P. Dieter and E. Fitzke, Formation of diacylglycerol, inositol phosphates, arachidonic acid and its metabolites in macrophages, Eur. J. Biochem. 218: 753 (1993).

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Biochemie und Molekularbiologie, Klinik für Tumorbiologie, Breisacherstr. 117, D-79106, Freiburg, Germany

    P. Ambs, E. Fitzke & P. Dieter

  2. INSERM U. 348, Hôpital Lariboisière 8, rue Guy Patin, 75475, Paris cedex, 10, France

    C. Créminon & J. MacLouf

Authors
  1. P. Ambs
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. Fitzke
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. Créminon
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. MacLouf
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. P. Dieter
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. The George Washington University Medical Center, Washington, D.C., USA

    Jack Y. Vanderhoek

Rights and permissions

Reprints and permissions

Copyright information

© 1996 Springer Science+Business Media New York

About this chapter

Cite this chapter

Ambs, P., Fitzke, E., Créminon, C., MacLouf, J., Dieter, P. (1996). Lipopolysaccharide-, Liposome-Encapsulated MTP-PE- and Dexamethasone- Regulated Prostaglandin Release in Rat Liver Macrophages: Role of Cytosolic Phospholipase A2 and Prostaglandin H Synthase-2. In: Vanderhoek, J.Y. (eds) Frontiers in Bioactive Lipids. GWUMC Department of Biochemistry and Molecular Biology Annual Spring Symposia. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5875-0_33

Download citation

  • DOI: https://doi.org/10.1007/978-1-4615-5875-0_33

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-7694-1

  • Online ISBN: 978-1-4615-5875-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation