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Cloning and Expression of cDNA Encoding Bovine Liver Dihydrodiol Dehydrogenase 3, DD3

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Enzymology and Molecular Biology of Carbonyl Metabolism 6

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 414))

Abstract

Dihydrodiol dehydrogenase (DDH) [EC 1.3.1.20] has been suggested to play an important physiological role in the metabolic detoxication of polycyclic aromatic hydrocarbons (PAHs) (Glatt et al., 1979). The pathways for metabolic activation and detoxication of a typical PAH, benzo[a]pyrene, are shown in Scheme 1. Environmental PAH is mainly activated through the action of liver P-450. The resulting 7,8-epoxide moiety is hydrolyzed by epoxide hydrolase in microsome. Then, the 7,8-dihydrodiol of PAH is further oxygenated to the 7,8-dihydrodiol-9,10-epoxide form by P-450 (Penning, 1993). This compound is assumed to be an ultimate carcinogen which binds covalently to DNA. Alternatively, DDH is reported as an important converting enzyme of the 7,8-dihydrodiol moiety to less active 7,8-dione (dicarbonyl). The dicarbonyl compound is further detoxicated with nonenzymatic ghlutathione-conjugation reaction (Penning, 1993).

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Japan

    Tomoyuki Terada, Hideki Adachi, Hirofumi Nanjo, Naomi Fujita, Jun-ichi Nishikawa, Masayoshi Imagawa, Tsutomu Nishihara & Masatomo Maeda

  2. Genome Information Research Center, Osaka University, 3-1 Yamada-oka, Suita Osaka, 565, Japan

    Tatsuya Takagi

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  1. Tomoyuki Terada
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  2. Hideki Adachi
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  3. Hirofumi Nanjo
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  6. Jun-ichi Nishikawa
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  7. Masayoshi Imagawa
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  8. Tsutomu Nishihara
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Editors and Affiliations

  1. Purdue University, West Lafayette, Indiana, USA

    Henry Weiner

  2. University of South Dakota School of Medicine, Vermillion, South Dakota, USA

    Ronald Lindahl

  3. Indiana University School of Medicine, Indianapolis, Indiana, USA

    David W. Crabb

  4. Queen’s University, Kingston, Ontario, Canada

    T. Geoffrey Flynn

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© 1996 Springer Science+Business Media New York

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Terada, T. et al. (1996). Cloning and Expression of cDNA Encoding Bovine Liver Dihydrodiol Dehydrogenase 3, DD3. In: Weiner, H., Lindahl, R., Crabb, D.W., Flynn, T.G. (eds) Enzymology and Molecular Biology of Carbonyl Metabolism 6. Advances in Experimental Medicine and Biology, vol 414. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5871-2_62

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  • DOI: https://doi.org/10.1007/978-1-4615-5871-2_62

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-7692-7

  • Online ISBN: 978-1-4615-5871-2

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