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Enzymology and Molecular Biology of Carbonyl Metabolism 6 pp 19–27Cite as

Human Corneal and Lens Aldehyde Dehydrogenases

Purification and Properties of Human Lens ALDH1 and Differential Expression as Major Soluble Proteins in Human Lens (ALDH1) and Cornea (ALDH3)

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 414))

Abstract

Aldehyde dehydrogenases (ALDHs) are now recognised as a complex gene family., which includes a group of NAD-dependent ALDH (EC 1.2.1.3) isozymes. Seven human ALDHs have been reported, of which ALDH1, 2, 3, 5, 6, and 7 (Hsu et al, 1994), and a related enzyme, γ-amino butyraldehyde dehydrogenase (GABADH) (Kurys et al, 1993), have been thus far cloned and sequenced (Hsu et al., 1994). Sequence analysis verifies that these are closely related enzymes, and optimised alignments show that 62 amino acids are conserved, including the catalytically significant Gly-245, Gly-250, Glu-268 and Cys-302 (Hsu et al., 1994). Human ALDHs 1, 2 and 3 have been further classified according to their genetic identity as Class 1(ALDH1, liver cytosolic), Class 2 (ALDH2, liver mitochondrial) and Class 3(ALDH3, stomach cytosolic) isozymes. In addition, a gene locus designated ALDHx, which shares 85% homology with Class 2 ALDH, has also been reported (Hsu and Chang, 1991).

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Authors and Affiliations

  1. Griffith University, Brisbane, Qld, 4111, Australia

    Gordon King

  2. The University of Newcastle, Callaghan, NSW, 2308, Australia

    Roger Holmes

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  1. Gordon King
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  2. Roger Holmes
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Editors and Affiliations

  1. Purdue University, West Lafayette, Indiana, USA

    Henry Weiner

  2. University of South Dakota School of Medicine, Vermillion, South Dakota, USA

    Ronald Lindahl

  3. Indiana University School of Medicine, Indianapolis, Indiana, USA

    David W. Crabb

  4. Queen’s University, Kingston, Ontario, Canada

    T. Geoffrey Flynn

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King, G., Holmes, R. (1996). Human Corneal and Lens Aldehyde Dehydrogenases. In: Weiner, H., Lindahl, R., Crabb, D.W., Flynn, T.G. (eds) Enzymology and Molecular Biology of Carbonyl Metabolism 6. Advances in Experimental Medicine and Biology, vol 414. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5871-2_4

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  • DOI: https://doi.org/10.1007/978-1-4615-5871-2_4

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-7692-7

  • Online ISBN: 978-1-4615-5871-2

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