Abstract
Differential distributions of alkaline phosphatase (AP) and dipeptydylpeptidase IV (DPPIV) were studied in coronary microvascular endothelial cells. Endothelial cells were obtained by the perfusion of coronary vessels with 0.1% trypsin PBS solution and cultured in uncoated culture dishes. Staining of cultured endothelial cells with AP- and DPPIVsensitive reagents revealed blue or red staining, respectively. Most colonies showed cells of only one color, blue or red, even at the fifth passage. AP-sensitive cells, which were originally elongated, shortened and widened, proliferating to form monolayer colonies of cobble stone-like cells. AP-stainability became weak with repeated passages. DPPIV-sensitive endothelial cells remained elongated even after repeated passages. The cell shape and stainability seemed to be coupled and maintained through the five passages studied.
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© 1997 Springer Science+Business Media New York
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Koyama, T. et al. (1997). Different Enzyme Activities in Coronary Capillary Endothelial Cells. In: Nemoto, E.M., et al. Oxygen Transport to Tissue XVIII. Advances in Experimental Medicine and Biology, vol 411. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5865-1_45
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DOI: https://doi.org/10.1007/978-1-4615-5865-1_45
Publisher Name: Springer, Boston, MA
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