Abstract
The autofluorescence of cancerous and normal mouse BALB/c strain tissues was measured in vitro, by fluorescence spectroscopy within three hours of surgery ablation. Three emission bands were observed in the 320-600 nm wavelength range.
For UVB excitation at 295 nm. one emission band is centered around 340 nm for cancerous tissue and 330 nm for normal tissue, For UVA excitation at 340 urn, the emission band for the cancerous mouse tissue, is centered about 400 nm and is practically absent for the normal mouse tissue when excited at the same wavelength. For both tissues a band centered at 470 nrn is also observed. In our previous study of the fluorescence spectroscopy of cancerous human stomach tissue we found an emission band centered around 380 urn that is practically absent in the normal tissue, when both tissues are excited at 340 urn, We found in this work with mouse tissue a very similar spectral behaviour concerning the differences between cancerous and normal human stomach tissues, with just some shift on the band centers and also some difference in the intensities, In an attempt to understand this behaiour we introduce some discussion about the tryptophan photophysics. and tryptophan metabolism, as the tryptophan amino acid seems to be the responsible for the fluorescence emission bands characteristics of the cancerous tissues.
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Rézio, T., Fraser Monteiro, M.L., Clode, W.H., Martinho, J.M.G. (1996). Fluorescence Spectroscopy for Diagnosis of Cancerous Tissues. In: Kohen, E., Hirschberg, J.G. (eds) Analytical Use of Fluorescent Probes in Oncology. NATO ASI Series, vol 286. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5845-3_42
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DOI: https://doi.org/10.1007/978-1-4615-5845-3_42
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