Abstract
In vivo models of myocardial infarction following coronary artery ligation in the rat still suffer from high early mortality and a low rate of success of myocardial infarction. This study investigated the possibility of reducing early mortality and increasing the rate of myocardial infarction by modifications of surgical techniques. Eighteen rats were divided into two groups: normal control (3 rats) and ligation (15 rats). The major modifications of surgical techniques used in this study include: (1) no exteriorization of the heart, (2) ligation of the origins of the branches rather than the main trunk of the left coronary artery, (3) removal of air from the chest after closure, (4) supplying oxygen immediately after extubation. Following surgery, the rats recovered uneventfully and 11 rats were alive after 16 weeks. One rat, with a large myocardial infarction, died 2 h after surgery. Early mortality (during surgery and 1 week after surgery) was 6.7% with a success rate of myocardial infarction of 85%. The left ventricle in the ligation group showed significant dilation relative to normal and sham-operated control hearts (317% of control hearts, p < 0.001). However, myocardial mass did not increase. The average infarct size was 33%. These results demonstrate that a reduction in early mortality and an increased success rate of myocardial infarction can be achieved by modifications of surgical techniques. (Mol Cell Biochem 176: 227-233, 1997)
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Johns TNP, Olson BJ: Experimental myocardial infarction. I. A method of coronary occlusion in small animals. Ann Surg 140: 675–682, 1954
Nachlas MM, Sieband MP: The influence of diastolic augmentation on infarct size following coronary artery ligation. J Thorac Cardiovasc Surg 53: 698–706, 1967
Maclean D, Fishbein MC, Maroko PR, Braunwald E: Hyaluronidase induced reductions in myocardial infarct size. Science 194: 199–200, 1976
De Tombe PP, Wannenburg T, Fan D, Little WC: Right ventricular contractile protein function in rats with left ventricular myocardial infarction. Am J Physiol 271: H73–H79, 1996
Yang XP, Sabbah HN, Liu YH, Sharow VG, Mascha EJ, Alwan I, Carretero OA: Ventriculographic evaluation in three rat models of cardiac dysfunction. Am J Physiol 265: H1946–H1952, 1993
Pfeffer MA, Pfeffer JM, Fishbein MC, Fletcher PJ, Spadaro J, Kloner RA, Braunwald E: Myocardial infarct size and ventricular function in rats. Circ Res 44: 503–512, 1979
Fishbein MC, Maclean D, Maroko PR: Experimental myocardial infarction in the rat. Am J Pathol 90: 57–70, 1978
Selye H, Bajusz E, Grasso S, Mendell P: Simple techniques for the surgical occlusion of coronary vessels in the rat. Angiology 11: 398–407, 1960 235-240
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1997 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Ye, J. et al. (1997). A new technique of coronary artery ligation: Experimental myocardial infarction in rats in vivo with reduced mortality. In: Singal, P.K., Panagia, V., Pierce, G.N. (eds) The Cellular Basis of Cardiovascular Function in Health and Disease. Developments in Molecular and Cellular Biochemistry, vol 22. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5765-4_29
Download citation
DOI: https://doi.org/10.1007/978-1-4615-5765-4_29
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-7642-2
Online ISBN: 978-1-4615-5765-4
eBook Packages: Springer Book Archive