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Effect of short-term treatment with a monoclonal antibody to P-selectin on balloon catheter-induced: Intimal hyperplasia, re-endothelialization, and attenuation of endothelial-dependent relaxation

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Book cover The Cellular Basis of Cardiovascular Function in Health and Disease

Part of the book series: Developments in Molecular and Cellular Biochemistry ((DMCB,volume 22))

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Abstract

The effects of an anti-P-selectin monoclonal antibody (MAb, PB 1.3; Cytel Corporation) on neoendothelialization; neoendothelial function, as evidenced by acetylcholine-induced relaxation (nitric oxide formation); and intimal hyperplasia following embolectomy catheter-induced injury to the rabbit thoracic aorta were investigated. Catheter injury was induced in two groups of New Zealand White rabbits. One group received no treatment, while the second group received short-term treatment with the MAb (i.p., immediately before and 12 h after induction of catheter injury). A third group underwent a sham operation and served as uninjured controls. Following sacrifice at 2 weeks after injury, aortic rings were assessed for degree of intimai hyperplasia, neoendothelial morphology (scanning electron microscopy), and acetylcholine-induced relaxation. Aortic tissue from catheter-injured animals that received treatment exhibited improved neoendothelial morphology, as compared with tissue from untreated but catheterized animals; however, no statistically significant attenuation of the hyperplastic response or improvement in the attenuated neoendothelial-dependent acetylcholine-induced relaxant response that is characteristic of neoendothelium that forms after catheter denudation was observed. These data suggest that short-term attenuation of P-selectin-mediated polymorphonuclear leukocyte (PMN)/endothelium, PMN/platelet interactions, and/or thrombin formation beneficially affects neoendothelialization of the vascular wall following balloon catheter-induced injury. (Mol Cell Biochem 176: 13–20, 1997)

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Author information

Authors and Affiliations

  1. Departments of Surgery, Tulane University School of Medicine, New Orleans, LA, 70112, USA

    Donald L. Akers & Brenda Bedi

  2. Departments of Medicine, Tulane University School of Medicine, New Orleans, LA, 70112, USA

    David J. Lefer, Robert G. Wilkens, Alan N. Tenaglia & Andrew J. Buda

  3. Departments of Anatomy, Tulane University School of Medicine, New Orleans, LA, 70112, USA

    I. Li Chen

  4. Departments of Pharmacology, Tulane University School of Medicine, New Orleans, LA, 70112, USA

    Harmeet Aurora, Thomas A. Osgood, Brenda Bedi, Philip J. Kadowitz & Dennis B. McNamara

  5. Departments of School of Tropical Health and Medicine, Tulane University School of Medicine, New Orleans, LA, 70112, USA

    Janet Rice

  6. Department of Pharmacology SL83, Tulane University School of Medicine, 1430 Tulane Avenue, New Orleans, LA, 70112, USA

    Dennis B. McNamara

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  1. Donald L. Akers
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Editors and Affiliations

  1. Institute of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, 351 Tache Avenue, Winnipeg, Manitoba, R2H 2A6, Canada

    Pawan K. Singal , Vincenzo Panagia  & Grant N. Pierce ,  & 

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© 1997 Springer Science+Business Media Dordrecht

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Akers, D.L. et al. (1997). Effect of short-term treatment with a monoclonal antibody to P-selectin on balloon catheter-induced: Intimal hyperplasia, re-endothelialization, and attenuation of endothelial-dependent relaxation. In: Singal, P.K., Panagia, V., Pierce, G.N. (eds) The Cellular Basis of Cardiovascular Function in Health and Disease. Developments in Molecular and Cellular Biochemistry, vol 22. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5765-4_2

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  • DOI: https://doi.org/10.1007/978-1-4615-5765-4_2

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-7642-2

  • Online ISBN: 978-1-4615-5765-4

  • eBook Packages: Springer Book Archive

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