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Chemical modification of an arginine residue in the ATP-binding site of Ca2+-transporting ATPase of sarcoplasmic reticulum by phenylglyoxal

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Muscle Physiology and Biochemistry

Part of the book series: Molecular and Cellular Biochemistry ((DMCB,volume 31))

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Abstract

Phenylglyoxal (PGO) was used as a reagent for chemical modification of the ATP-binding site of Ca2+-transporting ATPase of rabbit skeletal muscle sarcoplasmic reticulum (SR-ATPase). When 1 mM PGO was reacted with SR-ATPase at 30°C at pH 8.5, PGO was bound to the ATPase molecule in two-to-one stoichiometry with concomitant loss of activity of the ATPase to form the phosphorylated intermediate (E-P). ATP and ADP prevented the binding of PGO and thereby protected the enzyme from inactivation. The SR membranes were labeled with [14C]PGO and then digested with pepsin to identify the attachment site of PGO. A 14C-labeled peptide (402lle-Arg*-Ser-Gly-Gln406) was purified to homogeneity by C18-reversed phase HPLC (Arg* denotes the binding site of [14C]PGO). These results indicate that Arg403 is located in the ATP binding site of the SR-ATPase. (Mol Cell Biochem 190: 169–177, 1999)

*

Dedicated to Dr. Setsuro Ebashi who pioneered the field of Ca2+-mediated regulation of physiological processes.

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Authors and Affiliations

  1. Department of Physiological Chemistry, The Tokyo Metropolitan Institute of Medical Science (Rinshoken), Bunkyo-ku, Tokyo, Japan

    Hisanori Yamamoto & Masao Kawakita

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  1. Hisanori Yamamoto
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  2. Masao Kawakita
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Editors and Affiliations

  1. Department of Pharmacology, Niigata University School of Medicine, No. 757, Asahimachi-dori 1, Niigata 951, Japan

    Shoichi Imai

  2. Faculty of Medicine, Kyushu University, 812-82, Fukuoka, Japan

    Iwao Ohtsuki

  3. Saitama Medical School, 350-04, Moroyamamachi, Saitama, Japan

    Makoto Endo

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Yamamoto, H., Kawakita, M. (1999). Chemical modification of an arginine residue in the ATP-binding site of Ca2+-transporting ATPase of sarcoplasmic reticulum by phenylglyoxal. In: Imai, S., Ohtsuki, I., Endo, M. (eds) Muscle Physiology and Biochemistry. Molecular and Cellular Biochemistry, vol 31. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5543-8_21

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  • DOI: https://doi.org/10.1007/978-1-4615-5543-8_21

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-7534-0

  • Online ISBN: 978-1-4615-5543-8

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