Abstract
A total of 2,150 consecutive eyes enucleated from the deceased between 1 and 18 hours after death were obtained from various sources. Microbiological evaluation of the donor tissues before and after antiobiotic therapy was undertaken in all eyes. Positive cultures were obtained in 44.88% of the donor eyes; bacterial growth was seen in 41.06% of the eyes; and fungus noted in 3.81%. The number of pathogens isolated correlated directly with the death enucleation time. Staphylococcus albus was the most frequent bacterial isolate (37.03%), followed by Pseudomonas aeruginosa (14.72%). Antibiotics effective against most pathogens were Chloramphenicol and Framycetin. Polymyxin B was effective against Pseudomonas and cloxacillin against Staphylococcus. Thus, pre-enucleation instillation of framycetin (soframycin) eye drops in the donor eye followed by treatment of the donor eye ball with a 20 ml solution of normal saline containing 0.3 mg/ml of chloramphenicol for 10 minutes in the Eye Bank was recommended.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
B.W. Rycroft, The donor corneal graft. I Curso International de Oftalmologica 2:711 (1956).
J. Bobergans, Badsberg, E., and Rasmussen, J., Frequency of infection in donor eyes. Br. J. Ophthalmol. 46:365 (1962).
P.L. Farell, Fan, J.T., Smith, R.E., and Trousdale, M.D., Donor cornea bacterial contamination. Cornea 10:381 (1991).
A.S. Leveille, McMullian, F.D., and Cavanagh, H.D., Endophthalmitis following penetrating keratoplasty. Ophthalmology 90:38 (1983).
H.J. Rollins, and Stocker, F.W., Bacterial flora and preoperative treatment of donor corneas. Am. J. Ophthalmol. 59:247 (1965).
F.W. Polack, Khorazo, D.L., and Elizabeth G., Bacteriologic study of donor eyes. Arch Ophthalmol. 78:219 (1967).
G.J. Pardos, and Gallagher, M.A., Microbial contamination of donor eyes. Arch Ophthalmol. 100;1611 (1982).
T.G. Poole, and Michael S., Donor eyes contamination. Am. J. Ophthalmol. 97:560 (1984).
A. Panda, Angra, S.K., Venkateshwarlu, K., et al., Microbial contamination of donor eyes. Jpn. J. Ophthalmol. 32:264 (1988).
G. Satpathy, and Angra, S.K., Changing pattern of microbial contamination and antimicrobial sensitivity in donor eyes. Ann. Ophthalmol. 25:44 (1993).
J.C. Baer, Nirankari, V.S., and Glaros, D.S., Survival of Streptococcus viridans in gentamicin supplemented McCarey-Kaufman medium. Cornea 8:131 (1989).
A.P.G. Jose, Mohamad, R.D., Dua, H.S., et al., Positive donor rim culture in penetrating keratoplasty. Cornea 14:457 (1995).
J.D. Nelson, Mindrup, E.A., et al., and Ching, C.K., Fungal contamination in organ culture. Arch. Ophthalmol. 101:280 (1983).
M.S. Insler, and Urso, L.F., Candida albicans endophthalmitis after penetrating keratoplasty. Am. J. Ophthalmol. 104:57 (1987).
W. Behrens-Baumann, Ruechel, R., Zimmermann, O., and Vogel, M., Candida tropicalis endophthalmitis following penetrating keratoplasty. Br. J. Ophthalmol. 75:565 (1991).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1997 Springer Science+Business Media New York
About this chapter
Cite this chapter
Panda, A., Sharma, N., Angra, S.K., Kumbar, A. (1997). Microbial Contamination of Donor Eyes. In: Lass, J.H. (eds) Advances in Corneal Research. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5389-2_45
Download citation
DOI: https://doi.org/10.1007/978-1-4615-5389-2_45
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-7460-2
Online ISBN: 978-1-4615-5389-2
eBook Packages: Springer Book Archive