Abstract
Keratocytes play an important role in collagen metabolism in the corneal stroma. It has been reported that various cellular functions of keratocytes are regulated by extracellular matrices and cytokines. To understand the regulatory mechanism of collagenase synthesis by keratocytes, this study investigated the effects of extracellular collagen and cytokines on procollagenase synthesis by keratocytes. To perform this evaluation, subcultured human keratocytes were embedded in a type I collagen matrix. The cells were cultured for 24 hours with unsupplemented MEM. Various cytokines (IL-lβ, EGF, TGF-β, PDGF) were added to the medium. The amount of procollagenase in the medium was estimated by ELISA using a monoclonal antibody against human procollagenase. Results reveal that when keratocytes were cultured in a collagen matrix, the amount of procollagenase in the medium depended on either the incubation period or the number of cells. Furthermore, the amount of enzyme increased in proportion to the concentration of extracellular collagen. The amount of enzyme in the medium also increased by the addition of heat-denatured collagen. The addition of either IL-lβ, EGF, or PDGF stimulated procollagenase synthesis by the cells. On the other hand, enzyme synthesis was inhibited by the addition of TGF-β. The present results seem to indicate that procollagenase synthesis by keratocytes is regulated by extracellular collagen and various kinds of cytokines.
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Mishima, H., Abe, K., Otori, T. (1997). Regulatory Mechanism of Procollagenase Synthesis by Keratocytes. In: Lass, J.H. (eds) Advances in Corneal Research. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5389-2_36
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DOI: https://doi.org/10.1007/978-1-4615-5389-2_36
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