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Endothelial Sialyl Lewis X as a Crucial Glycan Decoration on L-Selectin Ligands

  • Risto Renkonen
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 435)

Abstract

Inflammatory reactions, such as organ transplant rejections, are characterized by lymphocyte infiltration into the tissue1. This extravasation of lymphocytes is initiated by the interaction of members of the selectin family and their ligands, which leads to a vascular shear flow-dependent rolling on the endothelial surfaces2–5. Of the three identified selectins, L-selectin is expressed on leukocyte surfaces and it recognizes glycoprotein ligands on endothelium6–8. Three characterized mucin-like heavily O-glycosylated proteins; GlyCAM-1, CD34 and MAdCAM-1 are endothelial L-selectin ligands9–11. These ligands recognize L-selectin only when posttranslationally glycosylated in a proper manner. So far only the crucial glycoforms of murine GlyCAM-1 have been characterized in great detail and they have been shown to be α2,3 sialylated, α1,3 fucosylated and sulfated, i.e. carry sialyl Lewis x (sLex) and/or sulfated sLex, respectively12–16. L-selectin was first characterized to guide lymphocyte traffic to lymph nodes and to sites of inflammation17. Today it is also known to participate in the rolling of leukocytes on vascular endothelium6–8. The two other members of selectin adhesion molecule family, E- and P-selectin, are expressed on activated endothelium3,18 and their glycoprotein ligands on leukocytes are active only when properly decorated with fucosylated oligosaccharides.

Keywords

Matrix Assisted Laser Desorption Ionisation Sialic Acid Capillary Endothelium Leukocyte Adhesion Deficiency Lymphocyte Homing 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Risto Renkonen
    • 1
  1. 1.Department of Bacteriology and Immunology Haartman InstituteUniversity of HelsinkiHelsinkiFinland

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