The Glycosylation of the Complement Regulatory Protein, Human Erythrocyte CD59
CD59 is a cell surface glycoprotein that binds to the complement proteins C8 and/or C9 in the nascent membrane attack complex, thereby protecting host cells from lysis (1). CD59 belongs to the Ly-6 superfamily (2) and is present on a wide variety of cell types, including leukocytes, platelets, epithelial and endothelial cells, placental cells and erythrocytes, where it is present at 2.5–5 × l04 copies/cell (3). In addition to its role in protecting host tissues from homologous complement, it has been proposed that CD59 mediates T-cell adhesive interactions by synergising with CD58 via direct interactions with CD2 (4,5) although this is controversial (6,7). It has also been proposed that CD59 participates in T cell activation pathways and platelet secretory responses (1).
KeywordsNormal Phase HPLC Elution Position Inositol Ring Exoglycosidase Digestion Biantennary Glycan
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