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Gene Therapy of Cancer pp 139–144Cite as

In Vivo Evaluation of a Drug-Inducible Vector System for the Combined Gene-and Chemotherapy of Cancer

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 451))

Abstract

Regulated expression of therapeutic genes in cancer gene therapy is used in a growing number of gene therapy approaches. Numerous conditionally active vectors have been tested carrying promoters which respond to certain factors or therapeutic modalities, such as irradiation, hyperthermia etc. [1].

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Abbreviations

FCS:

fetal calf serum

LTR:

long terminal repeats

MDR:

Multidrug Resistance

mdrl:

multidrug resistance gene

neo:

neomycin resistance gene

TE:

Tris-EDTA

TNF:

tumor necrosis factor

References

  1. W. Walther, U. Stein, 1996; Targeted vectors for gene therapy of cancer and retroviral infections. Mol. Biotechnol. 6, 267–286.

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  2. K. Kohno, S.-I. Sato, H. Takano, K.-I. Matsuo, M. Kuwano, 1989; The direct activation of human multidrug resistance gene (MDR1) by anticancer agents. Biochem. Biophys. Res. Comm. 165, 1415–1421.

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  3. U. Stein, W. Walther, R. H. Shoemaker, 1996; Vincristine induction of mutant and wild type human multidrug-resistance promoters is cell-type-specific and dose-dependent. J. Cancer Res. Clin. Oncol. 122, 275–282.

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  4. U. Stein, W. Walther, R. H. Shoemaker, 1996; Modulation of mdrl expression by cytokines in human colon carcinoma cells: an approach for reversal of multidrug resistance. Br. J. Cancer 74, 1384–1391.

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  5. U. Stein, W. Walther, R. H. Shoemaker, 1996; Reversal of multidrug resistance by transduction of cytokine genes into human colon carcinoma cells. J. Natl. Cancer Inst. 88, 1383–1392.

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  6. W. Walther, J. Wendt, U. Stein, 1997; Employment of the mdrl promoter for the chemotherapy-inducible expression of therapeutic genes in cancer gene therapy. Gene Ther. 4, 544–552.

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  7. G. Chu, H. Hayakawa, P. Berg, 1987; Electroporation for the efficient transfection of mammalian cells with DNA. Nucleic. Acids Res. 15, 1311–1326.

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Author information

Authors and Affiliations

  1. Max-Delbrück-Center for Molecular Medicine, Robert-Rössle-Straße 10, 13122, Berlin, Germany

    Dr. Wolfgang Walther, U. Stein, I. Fichtner & H. Naundorf

  2. National Cancer Institute, LDDRD, FCRDC, Frederick, Maryland, 21702, USA

    M. Alexander & R. H. Shoemaker

  3. Robert-Rössle-Clinic, UKRV, Lindenberger Weg 80, 13122, Berlin, Germany

    P. M. Schlag

Authors
  1. Dr. Wolfgang Walther
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  2. U. Stein
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  3. I. Fichtner
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  4. H. Naundorf
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  5. M. Alexander
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  6. R. H. Shoemaker
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  7. P. M. Schlag
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Editor information

Editors and Affiliations

  1. Humboldt University, Berlin, Germany

    Peter Walden , Uwe Trefzer , Wolfram Sterry  & Patricia Zambon , ,  & 

  2. King’s College School of Medicine and Dentistry, London, UK

    Farzin Farzaneh

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© 1998 Springer Science+Business Media New York

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Walther, W. et al. (1998). In Vivo Evaluation of a Drug-Inducible Vector System for the Combined Gene-and Chemotherapy of Cancer. In: Walden, P., Trefzer, U., Sterry, W., Farzaneh, F., Zambon, P. (eds) Gene Therapy of Cancer. Advances in Experimental Medicine and Biology, vol 451. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5357-1_22

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  • DOI: https://doi.org/10.1007/978-1-4615-5357-1_22

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-7444-2

  • Online ISBN: 978-1-4615-5357-1

  • eBook Packages: Springer Book Archive

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