Probing The Mechanisms Of Hepatocyte/Fibroblast Interactions
We have demonstrated that variations in tissue function can be achieved by modulation of spatial configuration of co-cultures of hepatocytes and fibroblasts. In Chapter 4 we showed that these differences in function were dependent on the degree of heterotypic interaction that occurred between cell populations. Furthermore, these findings correlated with the observation that hepatocellular islands of a critical size displayed spatial heterogeneity in the pattern of induction of hepatocyte differentiation- i.e. within a given hepatocyte population, hepatocytes close to the heterotypic interface demonstrated increased albumin production as compared to more central hepatocytes. Thus, evidence from markers of both bulk and localized liver-specific function suggested that the heterotypic interface between cell populations was an important determinant of the level of tissue function in the resultant tissue.
KeywordsSpatial Heterogeneity Tissue Engineer Urea Synthesis Hepatic Differentiation Issue Phase
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