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Triple Helix Forming Oligonucleotides pp 49–62Cite as

Imaging of Triplexes by Electron and Scanning Force Microscopy

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Part of the book series: Perspectives in Antisense Science ((DARE,volume 2))

Abstract

During the last decade numerous attempts have been made to use synthetic oligonucleotides, primarily triple-helix-forming oligonucleotides (TFO; 1–3), and more recently peptide nucleic acids (PNA; 4, 5) as tools for exploring DNA structure and for creating various methods for regulation of gene expression. TFOs and PNAs form triplexes with DNA, albeit of differing natures (6 and herein). It was assumed that an unusual parallel triplex, or R-DNA, is assembled as an intermediate structure during specific recombination mediated by RecA protein (7, 8). Genome analysis and labeling using various types of triplexes is an important microscopy project with great potential.

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Authors
  1. Dmitry Cherny
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Editors and Affiliations

  1. CNRS, France

    Claude Malvy , Annick Harel-Bellan  & Linda L. Pritchard ,  & 

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Cherny, D. (1999). Imaging of Triplexes by Electron and Scanning Force Microscopy. In: Malvy, C., Harel-Bellan, A., Pritchard, L.L. (eds) Triple Helix Forming Oligonucleotides. Perspectives in Antisense Science, vol 2. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5177-5_4

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  • DOI: https://doi.org/10.1007/978-1-4615-5177-5_4

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-7358-2

  • Online ISBN: 978-1-4615-5177-5

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