Comet Assay to Study UV-Induced DNA Damage

  • Éva Remenyik
  • Csaba Varga
  • Gabriella Emri
  • János Hunyadi
  • Irén Horkay

Abstract

The importance of environmental UV radiation is increasing. Its contribution to skin carcinogenesis has been widely accepted and intensively studied. One of the important steps in the biological effects of UV radiation is its DNA damaging effect. It has long been known that UV spectrum is separated in three different regions according to their biologic effect (UVC 100–280, UVB 280–320, UVA 320–410 nm). In the UVB spectrum the cyclobutane pyrimidine dimers and 6-4 photoproduct are the main DNA lesions. In higher wavelengths active oxygen species may be responsible for DNA strand breaks (1).

Keywords

Hydrogen Peroxide Agarose Electrophoresis Pyrimidine Cyclobutane 

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References

  1. 1.
    Anderson DYTW, Philips BJ, Schmezer P. The effect of various antioxidants and other modifying agents on oxygen-radical-generated DNA damage in human lymphocytes in the COMET assay. Mutat Res 1994:307:261-271PubMedCrossRefGoogle Scholar
  2. 2.
    Ostling O, Johanson KJ. Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochem Biphys Res Commun 1984:123:291-298CrossRefGoogle Scholar
  3. 3.
    Collins A; Dusinska M; Franklin M; Somorovska M; Petrovska H; Duthie S; Fillion L; Panayiotidis M; Raslova K; Vaughan N. Comet assay in human biomonitoring studies: reliability, validation, and applications. Environ-Mol-Mutagen. 1997:30: 139-46PubMedCrossRefGoogle Scholar
  4. 4.
    Collins AR; Dobson VL; Dusinska M; Kennedy G; Stetina R. The comet assay: what can it really tell us? Mutat Res. 1997:375:183-93PubMedCrossRefGoogle Scholar
  5. 5.
    Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantification of low levels of DNA damage in individual cells. Exp Cell res 1988:175:184-191PubMedCrossRefGoogle Scholar
  6. 6.
    Green MHL, Waugh APW, Lowe JE, Harcourt SA, Cole J, Arlen CF. Effect of deoxyribonucleosides on the hypersensitivity of human peripheral blood lymphocytes to UV-B and UV-C irradiation. Mutat Res 1994:315:25-32PubMedCrossRefGoogle Scholar
  7. 7.
    Alapetite C; Wachter T; Sage E; Moustacchi E. Use of the alkaline comet assay to detect DNA repair deficiencies in human fibroblasts exposed to UVC, UVB, UVA and gamma-rays.Int J Radiat Biol. 1996:69:359-69PubMedCrossRefGoogle Scholar
  8. 8.
    Lehmann J; Pollet D; Peker S; Steinkraus V; Hoppe U. Kinetics of DNA strand breaks and protection by antioxidants in UVA- or UVB-irradiated HaCaT keratinocytes using the single cell gel electrophoresis assay. Mutat Res. 1998:407: 97-108PubMedCrossRefGoogle Scholar
  9. 9.
    Lankinen MH; Vilpo LM; Vilpo JA. UV- and gamma-irradiation-induced DNA single-strand breaks and their repair in human blood granulocytes and lymphocytes. Mutat Res. 1996:352:31-8PubMedCrossRefGoogle Scholar
  10. 10.
    Peak JG, Pilas B, Dudek EJ, Peak MJ. DNA breaks caused by monochromatic 365 nm ultraviolet A radiation or hydrogen peroxide and their repair in human epitheloid and xeroderma pigmentosum cells. Photochemistry and Photobiology 1991:54:197-203PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1999

Authors and Affiliations

  • Éva Remenyik
  • Csaba Varga
  • Gabriella Emri
  • János Hunyadi
  • Irén Horkay

There are no affiliations available

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