Comet Assay to Study UV-Induced DNA Damage
The importance of environmental UV radiation is increasing. Its contribution to skin carcinogenesis has been widely accepted and intensively studied. One of the important steps in the biological effects of UV radiation is its DNA damaging effect. It has long been known that UV spectrum is separated in three different regions according to their biologic effect (UVC 100–280, UVB 280–320, UVA 320–410 nm). In the UVB spectrum the cyclobutane pyrimidine dimers and 6-4 photoproduct are the main DNA lesions. In higher wavelengths active oxygen species may be responsible for DNA strand breaks (1).
KeywordsHydrogen Peroxide Agarose Electrophoresis Pyrimidine Cyclobutane
Unable to display preview. Download preview PDF.