Comet Assay to Study UV-Induced DNA Damage

  • Éva Remenyik
  • Csaba Varga
  • Gabriella Emri
  • János Hunyadi
  • Irén Horkay


The importance of environmental UV radiation is increasing. Its contribution to skin carcinogenesis has been widely accepted and intensively studied. One of the important steps in the biological effects of UV radiation is its DNA damaging effect. It has long been known that UV spectrum is separated in three different regions according to their biologic effect (UVC 100–280, UVB 280–320, UVA 320–410 nm). In the UVB spectrum the cyclobutane pyrimidine dimers and 6-4 photoproduct are the main DNA lesions. In higher wavelengths active oxygen species may be responsible for DNA strand breaks (1).


Comet Assay HaCaT Cell Tail Moment Alkaline Comet Assay ISIS Software 
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Copyright information

© Springer Science+Business Media New York 1999

Authors and Affiliations

  • Éva Remenyik
  • Csaba Varga
  • Gabriella Emri
  • János Hunyadi
  • Irén Horkay

There are no affiliations available

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