All Three Vasopressin Receptor Sub-Types are Expressed by Small-Cell Carcinoma
We have demonstrated that vasopressin gene-related products are universal lineage markers of small-cell carcinoma of the lung (SCCL) and vasopressin is therefore most likely produced by all small-cell tumors1,2. This peptide has been found by others to promote tumor growth3,4. Such a mitogenic role, that involves Ca2+ mobilization and increases in MAP kinase activity, has been presumed by several investigators to be exercised through a single vasopressin receptor sub-type, namely the V1a receptor. In addition, a possible explanation advanced for the Ca2+ refractiveness to vasopressin of variant (dedifferentiated and drug-resistant) SCCL was a lost capacity to express this receptor5. However, we recently demonstrated that functional V1, receptors are present in both classical and variant SCCL6. We have now employed the techniques of RT-PCR, cloning, DNA sequencing, Northern analysis, and Western analysis, to examine the capacity of both classical and variant forms of SCCL to produce all three vasopressin receptor sub-types, namely V 1a receptors, V1b receptors, and V2 receptors.
KeywordsReceptor mRNA Nephrogenic Diabetes Insipidus Vasopressin Receptor Normal Human Lung Tumor Growth Inhibitory Effect
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