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Differential Effects of the Neurosteroid Pregnenolone Sulphate on Oxytocin and Vasopressin Neurones in Vitro

  • J. B. Wakerley
  • C. M. Richardson
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 449)

Abstract

Pregnenolone sulphate (PS) is a neurosteroid, synthesised within the brain, which has been reported to alter glutamatergic transmission primarily through allosteric interaction with NMDA receptors[1]. Previous work from this laboratory has shown that PS enhances NMDA-induced phasic firing in supraoptic vasopressin (VP) neurones[2]. The present study extends these initial findings by: (i) examining the effect of PS (100 µM) on firing evoked by the natural ligand, glutamate, in both supraoptic oxytocin (OT) and VP neurones and (ii) comparing the action of PS on NMDA- and AMPA-induced firing in these two cell types.

Keywords

Glutamatergic Transmission Supraoptic Nucleus Pregnenolone Sulphate Oxytocin Neurone Vasopressin Neurone 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Bowlby MR 1993 Pregnenolone sulfate potentiation of N-methyl-D-aspartate receptor channels in hippocampal neurons. Mol. Pharmacol. 43:813–819PubMedGoogle Scholar
  2. 2.
    Wakerley JB, Richardson, CM 1997 The neurosteroid, pregnenolone sulphate, enhances NMDA-induced phasic firing of vasopressin neurones in the rat supraoptic nucleus. Neurosci. Lett. 226:123–126PubMedCrossRefGoogle Scholar
  3. 3.
    Wakerley JB, Nobel R, Clark G 1983 Effects of morphine and D-ala, D-leu enkephalin on the electrical activity of supraoptic neurosecretory cells in vitro. Neuroscience 10: 73–81PubMedCrossRefGoogle Scholar
  4. 4.
    Wu FS, Gibbs TT, Farb DH 1991 Pregnenolone sulfate: a positive allosteric modulator at the N-methyl-Daspartate receptor. Mol. Pharmacol. 40:333–335PubMedGoogle Scholar
  5. 5.
    Majewska MD 1992 Neurosteroids: endogenous bimodal modulators of the GABAA receptor. Mechanism of action and physiological significance. Prog. Neurobiol. 38:379–395PubMedCrossRefGoogle Scholar
  6. 6.
    Richardson CM, Wakerley JB 1997 Glutamate excitation of oxytocin neurones in vitro involves predominantly non-NMDA receptors. Brain Res. 767:158–161PubMedCrossRefGoogle Scholar
  7. 7.
    van den Pol AN, Wuarin JP, Dudek FE 1990 Glutamate, the dominant excitatory transmitter in neuroendocrine regulation. Science 250:1276–1277PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • J. B. Wakerley
    • 1
  • C. M. Richardson
    • 1
  1. 1.Department of Anatomy, School of Medical Sciences, University WalkUniversity of BristolBristolUK

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